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The naturally occurring polyamines putrescine, spermidine, and spermine are found in all tissues and microorganisms (
1
–
5
). Polyamines are cations that have been implicated in various growth processes and in cellular differentiation (
6
). This chapter describes a simple and rapid method for the assay of polyamines. The method described here is based on that of Seiler (
7
) and enables one to determine 10–100 pmol of polyamines.