TLC identification of honeysuckle and two adulterants

Key words: Honeysuckle; adulterant; TLC identification of honeysuckle as a common Chinese medicine, mainly from the dry flower bud of Lonicera japonica Thunb In some areas, the flower buds of L hypoglauca Miq Are also used as honeysuckle for medicinal purposes In the process of application, it was found that L maackii (Rupr.) Maxim Had similar characters with honeysuckle, which was easily confused At the same time, the phenomenon that the flower of Daphne genkwa was used to be false honeysuckle occurred In order to ensure the accuracy and effectiveness of clinical medication, the author has carried out a comparative study on them by TLC Now the results are introduced as follows 1 The samples were crushed and sieved through 40 mesh Weigh 1g respectively, add 15ml of methanol, and soak for 4H at temperature The filtrate is volatilized to methanol in water bath, and 2ml of methanol is added to the residue for chromatography Chromatographic condition T: silica gel G (Qingdao) - 0.7% CMC Na, wet plate, activated at 105 ℃ for 0.5h The sample size is 6 μ L Developer: ethyl hexanoate: acetic acid (8:2) The exhibition distance is 12cm Detection method: fluorescence spots were observed at 365nm Results 2.1 TLC of honeysuckle and its adulterants 2.2 there are three spots in Lonicera japonica, RF value is 0.19, 0.4b and 0.78 respectively; there are three spots in Lonicera rubra, RF value is 0.18, o.45 and 0.80 respectively There are four spots in the flower of alchemy, with RF values of 0.13, 0.20, 0.44 and 0.88 respectively; there are 80 spots in the flower of Wuhu, with RF values of 0.o8, 0.15, o.19, 0.25, 0.31, 0.49, 0.75 and 0.90 respectively Discussion from the experimental results, we can see that the number, color and RF value of the fluorescent spots of Lonicera japonica and Lonicera rubra are basically the same, indicating that the ingredients are basically the same, so they are listed as honeysuckle in the Chinese Pharmacopoeia Although the color and RF value of two spots of honeysuckle flower are similar to that of honeysuckle flower, one light blue spot is added at RF value 0.13, and one red spot is added at RF value 0.88, which is easy to distinguish honeysuckle flower Daphne genkwa and honeysuckle are different in chemical composition, so the difference of TLC is significant and easy to identify In conclusion, TLC is a simple and effective method to identify honeysuckle and its adulterants.