 Home > Biochemistry News > Microbiology News > Transposon Mutagenesis in Clostridium difficile

Transposon Mutagenesis in Clostridium difficile

 Last Update: 2021-02-12
 Source: Internet
 Author: User

Tags

site saturation mutagenesis

site directed mutagenesis

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Genetic manipulation of
Clostridium difficile
is notoriously difficult, currently there is only one reliable method for generating random mutations in the organism and that is to use the conjugative transposon Tn
916
. Tn
916
enters the genome of most strains of
C. difficile
with no obvious target site preference. In order to use the genome strain
C. difficile
630 for transposon mutagenesis a erythromycin-sensitive derivative
C. difficile
630Δ
erm
was constructed and the Tn
916
derivative, Tn
916
ΔE, was shown to enter the genome at multiple sites enabling the construction of a Tn
916
insertion library.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

Novel coronavirus RNA nucleic acid quality control sample (low value) BNCC359801 BNCC

CAS No.:

$220-280/MT EXW

Fecal coliform negative control samples (filter method) BNCC359577 BNCC

CAS No.:

$220-280/MT EXW

Stendomycin (8CI,9CI)

CAS No.: 11006-78-3

$1/KG EXW

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

announcements today(81)

80 20 company(94)

united states logo(227)

single use technology(195)

healthy food name(42)

ups united states(221)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.