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Use of Self-Quenched, Fluorogenic LUX Primers for Gene Expression Profiling

 Last Update: 2020-12-20
 Source: Internet
 Author: User

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all time detection

real world application

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Application of a real-time detection system based on a novel primer design in gene expression profiling is described. In this system, called LUX
TM
(Light Upon eXtension), the generation of signal is based on a single fluorescent dye molecule that is attached to an oligonucleotide close to the 3′-end. A primer design software is available that identifies LUX primer pairs based on a set of rules for optimum signal development. The use of LUX fluorogenic primers to determine the expression patterns of various transcripts during differentiation in the P-19 mouse neuronal model is described.

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