What problems should be paid attention to in the use of biological safety cabinets?

What problems should be paid attention to in the use of biological safety cabinets? The biological safety cabinet is a common experimental equipment, so many things should be paid attention to in use
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Matters needing attention in the use of biological safety cabinets 3.
1 Management of personnel 3.
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1 Only trained and instructed laboratory staff can operate the equipment, and untrained personnel cannot use it
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2 Non-staff members are prohibited from entering the laboratory, and staff members are prohibited from smoking while operating the equipment and handling contact lenses
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3 After completing the operation, the staff should truthfully fill in the "Use Record"
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2 Management of equipment 3.
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1 Use a separate power supply for the biological safety cabinet to prevent the failure of the biological safety cabinet due to accidents caused by other equipment
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2 The vents on the top of the equipment must not be covered by anything to ensure smooth exhaust
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3 Stop using the equipment when an alarm occurs, and then use it after finding out the reason to ensure safety
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If there is a signal of system failure, the device cannot continue to operate because the air cannot be filtered
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4 When replacing the air filter, the engineer of the manufacturer should be contacted and replaced.
The laboratory staff cannot replace it without permission, so as to avoid pollution to the surrounding personnel and the environment caused by improper operation
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5 The used filter should be placed in a closed container, and it should be noted in a clear location that it may contain dangerous microorganisms, and it should be noticed and disposed
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6 The overall performance of the newly purchased biological safety cabinet shall be certified by professional technicians before use or after maintenance and relocation.
Each biological safety cabinet shall be tested and certified at least once a year to ensure the operation of the cabinet.
Safety
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3.
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7 The performance test of the biological safety cabinet should include: the test of the speed and volume of the air supply air, the test of the air inlet speed, the test of the airflow and smoke flow pattern, the test of the penetration of the air filter, the test of the leakage of the safety cabinet, the leakage of electrical appliances and the grounding resistance And polarity test, light intensity test, vibration test, noise level test, UV lamp test,
etc.

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3 Preparations before work 3.
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1 Before starting work, let the biological safety cabinet sealing machine run for at least 3-5 minutes to purify the air in the safety cabinet
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2 Wipe the work surface, inner wall and inner surface of the observation window of the safety cabinet with disinfectant, and then wipe with sterile distilled water to remove the residual disinfectant
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3 The surfaces of containers and materials placed in the biological safety cabinet should be wiped with 75% alcohol to reduce the introduction of environmental bacteria into the safety cabinet
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Precautions in operation 4.
1 The operator can only operate the biological safety cabinet after he has done personal protection
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2 The operator should put the arm into the biological safety cabinet for about one minute before performing the experimental operation, which can stabilize the safety cabinet and allow the air flow to clean the microbial contamination on the surface of the arm
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3 All operations should be performed on a work surface within four inches of the front grille, which must not be obstructed by anything
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4 All materials should be placed as far as possible from the grille in front of the safety cabinet and the edge of the work surface at the back.
Equipment that is prone to aerosols (such as agitators, centrifuges, etc.
) should be placed close to the rear of the safety cabinet
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5 The movement during operation should be from the clean area of ​​the work surface to the contaminated area, generally from the left to the right (the opposite for those who are accustomed to left-handed operation)
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6 If a small amount of spray occurs in the biological safety cabinet, use the pollution-absorbing tissue to deal with it immediately, put the used tissue in the biohazard bag, and then wipe and disinfect immediately with a towel soaked in disinfectant.
In case of overflow, extensive disinfection is required.
All items in the safety cabinet should be surface-sterilized, and the safety cabinet should be taken out.
The action time of the disinfectant should be maintained for 20-30 minutes
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7 It is forbidden to use alcohol lamps in the biological safety cabinet.
The use of alcohol lamps to disinfect and inoculate tools is prone to danger, and electronic high-temperature sterilization equipment should be used
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8 When the work is completed, all containers and equipment should be surface disinfected and the safety cabinet should be taken out.
Wipe and disinfect the countertop, edge, rear wall and the inside of the observation window of the safety cabinet
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4.
9 Staff should take off gloves, take off work clothes and wash their hands when leaving the laboratory.
The above is the relevant introduction to the use of biological safety cabinets.
I believe that everyone has a corresponding understanding of this after reading it.
You can use it as a reference.
helped
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