What should be paid attention to when using biological safety cabinets?

The prevention of biological contamination during biological experiments is also an important part of biological laboratory safety
.

Its role is to effectively protect the safety of laboratory personnel and the laboratory environment
.

So what should we pay attention to when using it? 1.
When using the biological safety cabinet, wear personal protective clothing and gloves to prevent contamination
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Ordinary laboratory uniforms may be worn during Class I and Class II biosafety operations
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Gloves should be worn at the lab cuff
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Masks and goggles may also be required for some operations
.

2.
Avoid using open flame sterilizers such as alcohol lamps in the biological safety cabinet
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Using an open flame can affect airflow and handle volatile, flammable materials, but can also be dangerous
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In the sterilization of inoculation loops, an infrared heater can be used, or a disposable plastic inoculation loop can be simply used
.

3.
Put the hands and arms into the biological safety cabinet and wait for about 1 minute to adjust the safety cabinet and "sweep" the air on the surfaces of the hands and arms before starting to handle the items
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　　　　4.
Minimize arm movements during operation, and move slowly to avoid disturbing the airflow in the cabinet
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When entering and exiting the safe, take care to maintain the integrity of the airflow at the opening
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The arms should be perpendicular to the opening area and move slowly in and out of the front opening
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　　　　5.
The operation of the tabletop experiment should be carried out from the clean area to the contaminated area, and all operations are carried out in the middle and rear of the tabletop
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　　　　6.
When working, the biohazardous waste bag and the autoclave-resistant suction cup should be put into the safety cabinet, and should not be taken out of the safety cabinet
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Otherwise, when using these items, the arms should be in and out of the cabinet frequently, which may interfere with the integrity of the cabinet's gas barrier and affect the protection of people and items
.

　　　　7.
If there is leakage during the experiment, use sterile paper towel on the surface to absorb and clean
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　　　　8.
Try to keep the biological safety cabinet in a continuous working state, and disinfect all items to maximize the protection of personnel
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Surfaces must be disinfected when removing potentially contaminating items from the cabinet
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