Why does the centrifuge tube break when centrifuges?
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Last Update: 2020-10-26
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Source: Internet
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Author: User
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asked:
digestion
filter after centrifugal, 3000 s/min 15 minutes,
centrifuge tube
broken, why? Has it anything to do with centrifuge speed? Please help with the analysis.
Answer; if you're not mistaken, you're using a vertical
centrifuge
, which I don't think has much to do with a angle velocity and centrifugal force, maybe the 2-3 centrifuges you place in a large centrifugal cup when you're several tubes away at a time, caused by the centrifugal force squeezing each other, or the rubber pads in the large centrifuges aging hardening and squeezing the centrifuges when centrifuges are centrifugal. It is recommended that when centrifuging, 800-1000rpm, 5min can leave the cells, or insert centrifuges into the large centrifuge cup without too much effort, gently put it in.
: Our laboratory is such a situation: centrifugation set time 5min, if not artificially make it stop, it will start to rise after 5min from the original set speed, and the glass centrifuge tube is up to 3000 l/min, beyond it is easy to break, so we often choose to use plastic centrifuge tube when centrifugation 3000. I don't know if that's the case with your lab centrifuges, I suggest you use plastic centrifuges.
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