Zhang peace team to crack the mother-to-child microbiotic transmission mechanism

Original title: Basic Research . . Zhang Peace Team cracks the mother-to-child microbiotic transmission mechanism The major basic research opening topics in our region add new achievements Recently, the autonomous region's major basic research open topics undertaken by the team of Professor Zhang Peace of Inner Mongolia Agricultural University, "mother-to-child bacteria transmission mechanism and correlation research" through expert acceptance.gut microorganisms are closely related to human health. Early studies have suggested that babies are born sterile and that the creation of the baby's gut bacteria is influenced only by bacteria in the surrounding environment. The project is designed to gain insight into the origin of early gut microbes in infants and to analyze the mechanism of transmission of maternal and child virlobis.
Pictured: The mother source of the fetal fecal microbiome. (a) The total number of OTUs in the fetal fecal microbiome and the maternal microbiome. (b) The genus of bacteria common to the fetal fecal microbiome and the mother microbiome. (c) A genus of bacteria in fetal feces that is common to only one mother sample. IF: Fetal feces; AF: amniotacus; MF: maternal feces; MS: maternal saliva; MV: vaginal fluid
.the team used a combination of three generations of sequencing and microdrop digital PCR to study the bacterial structure of mothers and babies using 41 pairs of female and child volunteer feces, mother's feces, amniotic fluid, birth channel fluid, breast milk and maternal saliva samples. The results showed that fetal feces, amniotic fluid and breast milk had the advantage of thick-walled bacteria and deformed bacillus, while mother's feces and saliva had the advantage of thick-walled bacteria and bacillus, indicating that fetal feces and amniotic fluid and breast milk had more similar bacterial structure. Analysis of bacteriosophology similarity based on OTU levels found that fetal feces and amniotic fluid, vaginal fluid, breast milk cytosus similarity is significantly higher than other maternal source samples, indicating that the intestinal bacteriostructure structure of early infants is closely related to their pre-birth living environment (amniotic fluid) and temporary living environment (birth pathway) during childbirth, the fetus may be in the womb by swallowing amniotic fluid to obtain the original mother-source microorganisms.The team found through a micro-drip digital PCR analysis that the absolute content of lactobacillus genus in the fetal feces of a naturally delivered baby is significantly higher than that of babies delivered by caesarean section, indicating that different delivery methods affect the planting of Lactobacillus in the early intestines of the baby, which may be related to the birth of the baby during natural delivery, resulting in bacterium transmission.
the project revealed that the baby fetal fecal microorganisms are transmitted from multiple maternal sites, of which the amniocal microbiome contributes the most to the fetal fecal microbiome. This provides an important reference for understanding the origin of human gut microorganisms.(Basic Research Service)