chlamydomonas reinhardtii

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Electroporation of DNA into the Unicellular Green Alga Chlamydomonas reinhardtii

 Time of Update: 2021-01-16

Recently developed techniques for efficient transformation ( 2 – 8 ) and insertional mutagenesis ( 9 ) ofChlamydomonas nuclear and organelle genomes promise to accelerate the genetic analysis of these and other complex cellular processes.
Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii

 Time of Update: 2021-01-15

The genetic transformation ofChlamydomonas is relatively simple and efficient, but achieving high expression levels of foreign genes has remained challenging. In addition, we describe available tools for promoting efficient transgene expression and highlight important considerations for designing transformation vectors.
A Simple Method for Chloroplast Transformation in Chlamydomonas reinhardtii

 Time of Update: 2020-11-08

Chlamydomonas reinhardtii is also a favorable organism for chloroplast transformation because it contains a single chloroplast and grows heterotrophically when supplemented with acetate. Chlamydomonas has served as a model organism for the development of chloroplast transformation procedures and the study of photosynthetic mutants generated using this method.

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