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SSCP Analysis of Point Mutations by Multicolor Capillary Electrophoresis
Time of Update: 2021-01-02
Virtually all methods for the detection of mutations (polymorphism or variant) rely on polymerase chain reaction ( PCR ).
Techniques to detect variants as positive signals have the advantage of intrinsically low false positive rate, and are suitablemethods to preselect fragments that carry mutations among an excess of nonmutated fragments.
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Analysis of Short Tandem Repeat Markers by Capillary Array Electrophoresis
Time of Update: 2020-12-29
The regionof this gene that expands to disease-causing mutations consists of two adjacent tandemly repeated polymorphic triplet repeats:a diagnostic (CAG)n repeat immediately followed by a (CCG)n region.
Early polymerase chain reaction ( PCR )-based methods usedto genotype disease-causing mutations amplified through both of the repeat regions.
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Analysis of DNA Damage Using Capillary Zone Electrophoresis and Electrospray Mass Spectrometry
Time of Update: 2020-12-28
However, the focus of this chapter is on the analysis of the products resulting from this DNA damage and the DNA adducts by capillary zone electrophoresis coupled on-line to electrospray mass spectrometry (CZE-ESI-MS).
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Differential Display Analysis by Capillary Electrophoresis
Time of Update: 2020-12-27
Differential display polymerase chain reaction (DD- PCR ), originally described by Liang and Pardee in 1992 ( 1 ), has been utilized to study gene expression events in a myriad of biological processes.
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Monosaccharide Analysis by Capillary Electrophoresis
Time of Update: 2020-12-27
All carbohydrates contain hydroxyl group and most of them in addition havea characteristic functional group, such as the hemiacetal (aldoses), keto (ketoses), and carboxyl (aldonic and saccharic acids)groups.
Each of the aforementioned groups comprises homologues havingdifferent numbers of carbon atoms.
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High Resolution Analysis of Point Mutations by Constant Denaturant Capillary Electrophoresis (CDCE)
Time of Update: 2020-12-24
At one extreme, CDCE is capable of detecting mutants at very low frequencies (as lowas 10 -6 ) ( 2 , 3 ), in which it rivals the high sensitivity mutation detection methods like allele specific PCR.
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Capillary Electrophoresis-Mass Spectrometric Analysis of DNA Adducts
Time of Update: 2020-12-20
The compounds, which result from a covalent attachment of the chemical with DNA, are called “DNA adducts.” Because of their potential for initiating cancer, the characterization and quantitation of DNA adducts are important.
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Capillary Electrophoresis in the Analysis and Monitoring of Biotechnological Processes
Time of Update: 2020-12-18
We have applied capillary zone electrophoresis (CZE) andSDS -gel CE for the characterization of recombinant proteins during development of major bioprocessing steps, including fermentation, hybridoma cell cultivation, chromatographic purification, and chemical transformation.
Rapid SDS-gel CE was developed for the fast analysis of fermentation broth and hybridoma cell culture.
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Gel-Capillary Electrophoresis Analysis of Oligonucleotides
Time of Update: 2020-12-16
Gel-capillary electrophoresis is a new alternative to traditional electrophoretic techniques for the analysis of oligonucleotides ( 1 – 5 ).
Slab gels (PAGE), on the other hand, are visualized at a single time point in the analysis, do not easily yield to integrative quantitation, and are thus asubjective method of analysis.
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Capillary Electrophoresis-Mass Spectrometry for Peptide Analysis: Target-Based Approaches and Proteomics/Peptidomics Strategies
Time of Update: 2020-12-16
Namely, CE-MS using an ion trapMS analyzer is applied to detect and quantify γ-glutamyl- S -ethenyl-cysteine (GEC) bioactive dipeptide in a legume plant.
These generic methods have broadapplications for the analysis of peptides in a large variety of matrices, including applications in the area of proteomicsand peptidomics.
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Glycosaminoglycans: Oligosaccharide Analysis by Liquid Chromatography, Capillary Electrophoresis, and Specific Labeling
Time of Update: 2020-12-15
Glycosaminoglycans (GAGs) are a class of biopolymers that include chondrotin sulfate, dermatan sulfate, keratan sulfate, hyaluronic acid, heparin, and heparan sulfate.
The GAGs are linear polysaccharides that are microheterogeneous in composition and polydisperse in size.
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Development of a High-Throughput Capillary Electrophoresis Protocol for DNA Fragment Analysis
Time of Update: 2020-12-14
The first type of matrix is of high viscosity polymer (e.g.,polyacrylamide) with a well-defined crosslinked gel in regard to the structure and size of its pores.
Many differentmedia useful for the separation of DNA have now become commercially available ( 6 ).
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Application of Capillary Electrophoresis to Pharmaceutical Analysis
Time of Update: 2020-12-12
The majority of drugs are either acidic or basic water-soluble compounds, and many drugs may be adequately separated by FSCE.
Figure 1 shows the efficient separation of a test mixture containing 11 basic drugs using a simple low-pH electrolyte.
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Analysis of Bases, Nucleosides, and (Oligo)nucleotides by Capillary Electrophoresis
Time of Update: 2020-12-09
In this chapter, first CZE and MEKC examples of bases, nucleosides, and nucleotides are discussed in which UV absorbance is used as the detection method.
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Highly Charged Polyelectrolyte Coatings to Prevent Adsorption During Protein and Peptide Analysis in Capillary Electrophoresis
Time of Update: 2020-12-09
The use of non-covalent coatings using highly charged polyelectrolytesis an efficient, simple, and fast approach to reduce peptide and protein adsorption phenomena.
When optimal conditions defined in the protocolsare used, very stable coatings are obtained and adsorption phenomena are eliminated.