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PNAS: Adjacent cell labeling technology based on membrane-permeable fluorescent proteins
Time of Update: 2023-02-03
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Neuron Luo Liqun/Yu Anyang/Li Jiefu et al. developed in situ labeling methods for mammalian cell surface proteomes
Time of Update: 2022-10-21
Subsequently, this year, the team used this technique to discover the cell surface protein combination coding pattern controlled by transcription factors (see BioArt report: Neuron | Luo Liqun/Xie Qijing/Li Jiefu et al.
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Application of iTRAQ/TMT labeling quantitative proteome in the study of new understanding of non-small cell carcinoma metastasis mechanism
Time of Update: 2022-08-30
Article Title: Profilin 1 Induces Tumor Metastasis by Promoting Microvesicle Secretion Through the ROCK 1/p-MLC Pathway in Non-SmallCell Lung Cancer Published journal: Frontiers in Pharmacology Release time: 2022.
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ARD: Baricitinib in recurrent giant cell arteritis: a 52-week prospective open-label pilot study
Time of Update: 2022-08-16
Methods We conducted a prospective, open-label pilot study of baricitinib ( 4 mg/ day) with graded glucocorticoids ( GC ) and accelerated tapering in patients with recurrent GCA .
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Nature Explains Important Cell Modification Process: Key Steps in Adding Chemical Labels to Transfer RNA
Time of Update: 2021-10-01
A new study by researchers at Penn State University reveals the chemical steps involved in adding an important tag—methyl sulfur group to transfer RNA.
Imaging the effects of MiaB and SAM molecules and tRNA at several points during the methylthiolation process allows researchers to infer the chemical steps in the modification process .
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Lancet Rheumatology: Tocilizumab monotherapy for giant cell arteritis after ultra-short
Time of Update: 2021-09-18
Trial recruited 50 over the age of new diagnostic giant cell arteritis ( screening prior to Visit 4 American College of Rheumatology criteria or big weeks) in line with the vessel vasculitis polymyalgia rheumatica patients .
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Use small molecule protein complexes to label stem cells to achieve high spatiotemporal resolution in vivo tracing
Time of Update: 2021-08-28
Recently, Cheng Zhen and Chen Hao’s team from the Molecular Imaging Center of Shanghai Institute of Materia Medica, Chinese Academy of Sciences collaborated with the research group of Associate Professor Li Kai from Southern University of Science and Technology to design a stem cell tracer (CelTrac1000) based on the NIR-II small molecule dye protein complex.
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Selectable labeling of mitochondrial proteins through visible light-catalyzed reactions in living cells
Time of Update: 2021-08-27
Recently, Chen Yiyun’s group at the Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences collaborated with Zhang Yaoyang’s group (intersection center) to take advantage of the high temporal and spatial resolution of light, and for the first time to use organic small molecule dyes as biocompatible photocatalysts for subcellular localization.
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Choline Labeling and TNF Treatment of HL-60 Cells
Time of Update: 2021-03-07
3) Resuspend final pellet in an appropriate volume of prewarmed serum free media and supplement with ITS (4 ml/L should yield 5 mg/L insulin and 5 mg/L transferrin).
6) Wash out label by pelleting cells and very gently washing 1 time with room temperature PBS.
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On-Column Labeling Reaction for Analysis of Protein Contents of a Single Cell Using Capillary Electrophoresis With Laser-Induced Fluorescence Detection
Time of Update: 2021-03-05
This chapter presents methods for capillary electrophoresis (CE) fingerprinting of proteins in a cell extract and in single cells.
More than 20 relevant publications are cited in this chapter to assist the reader with adopting the presented methods.
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Phosphoinositidase C Activation Assay I: Cell Labeling, Stimulation, and Recovery of Cellular [3H]Phosphoinositides and [3H]Phosphoinositols
Time of Update: 2021-02-24
For phosphoinositols, abbreviations are in the form Ins(x,y,z )Pn , wherex, y , andz refer to the positions of the phosphate groups on the myo-inositol ring andn refers to the total number of phosphates.
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Solve the mystery of cancer metastasis CRISPR technology gives each cancer cell a special "label"
Time of Update: 2021-02-11
Using single-cell RNA sequencing, the researchers compared gene expression maps of cancer cells with different metastasis potentials.
, they can affect the metastasis potential of cancer cells when researchers use CRISPR technology to reduce or increase the level of expression of these genes in another lung cancer cell line.
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The Role of Inositol and the Principles of Labelling, Extraction, and Analysis of Inositides in Mammalian Cells
Time of Update: 2021-01-06
In this chapter, we establish a broad foundation for the rest of the book by covering some important principles of inositide methodologies.
The focus is especially directed to those methods or aspects of methodology not covered in detail in other chapters.
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Single-Step Conjugation of Antibodies to Quantum Dots for Labeling Cell Surface Receptors in Mammalian Cells
Time of Update: 2021-01-05
Labeling of cell surface receptors in living cells can be achieved using antibody-conjugated semiconductor quantum dots (QDs).
We describe herein methods that allow conjugation of antibodies to QDs in a single step without the formation of side products.
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Stable Isotope Labeling by Amino Acids in Cell Culture for Quantitative Proteomics
Time of Update: 2020-12-29
Stable isotope labeling by amino acids in cell culture (SILAC), one of the more widely used methodsfor quantitative proteomics, is a metabolic-labeling strategy that encodes whole cellular proteomes.
Incorporation of the “heavy” amino acid occurs through cell growth, protein synthesis, and turnover.