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Studying Interactions Between Chloroplast Proteins in Intact Plant Cells Using Bimolecular
Time of Update: 2021-01-17
Protein–protein interactions play crucial roles in the execution of many cellular functions, including those in plastids.
Identifying and characterising protein–protein interactions can yield valuable information regarding the function of a protein and can also contribute towards understanding protein–protein interaction networks in plastids, thereby contributing to a better understanding of cellular processes.
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Membrane and Protein Dynamics in Virus-Infected Plant Cells
Time of Update: 2021-01-16
Therefore, to meet the challenges of diverse cell biology studies that will be derived from ongoing functional genomics projects, a series of methods relevant to the characterization of membrane and protein dynamics in virus-infected cells are provided here.
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Large-Scale Culture of Plant Cells
Time of Update: 2021-01-16
It was soon found, however, that although plant cell suspensions appear to be similar in many ways to microbial cultures, there are, in fact, key differences that can have a significant influence on large-scale cultivation.
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Electrofusion of Plant Cells
Time of Update: 2021-01-16
Electrofusion offers definite advantages over more commonly used protocols that make use of chemical stimulation to induce fusion.
Additionally, fusion events can be monitored microscopically, allowing the precise determination of the effects of various electrical parameters and, therefore, the use of optimum electrical values.
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Movement Profiles: A Tool for Quantitative Analysis of Cell-to-Cell Movement of Plant Viral Movement Proteins
Time of Update: 2021-01-16
Expressing cells can be directly monitored for subcellular localization and cell-to-cell movement of the MP:GFP fusion protein into neighboring cells by confocal scanning microscopy.
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Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy
Time of Update: 2021-01-14
Histochemical methods allow for identification and localization of various components within the tissue.
Such information on the spatial heterogeneity is not available with biochemical methods.
Hereby we present set of selected simple staining and histochemical methods with comments based on our laboratory experience.
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Heterologous Expression in Budding Yeast as a Tool for Studying the Plant Cell Morphogenesis Machinery
Time of Update: 2021-01-13
The budding yeast ( Saccharomyces cerevisiae ) can serve as a unique experimental system for functional studies of heterologous genes, allowing not only complementation of readily available yeast mu
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Suspension-Cultured Plant Cells as a Tool to Analyze the Extracellular Proteome
Time of Update: 2021-01-13
Despite the central role played by extracellular proteins in most processes that control growth and development, the secretome has been less well characterized than other subcellular compartments, meaning that our understanding of the cell wall physiology is still very limited.
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Mechanical Measurements on Living Plant Cells by Micro-indentation with Cellular Force Microscopy
Time of Update: 2021-01-13
Indentation methods on the micro- and nanoscale are increasingly used to assess mechanical properties of living plant tissues.
Cellular force microscopy is a micro-indentation method that was designed to precisely measure and apply forces on living plant cells.
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Characterization of the Plant Cell Wall Proteome Using High-Throughput Screens
Time of Update: 2021-01-13
Plant cell wall proteins play essential roles in many important biological processes, and yet there is still not a comprehensive catalogue of the cell wall proteome, or “secretome”.
The approaches are orthogonal and collectively provide a powerful suite of approaches to complement more commonly used strategies to isolate plant cell wall-associated proteins.
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The omnipotence of plant cells
Time of Update: 2021-01-10
However, their genetic potential has not been lost, all genetic information is still maintained in the sequence of DNA, once out of the original organ tissue, become free state, in a certain nutritional conditions and plant monucogen induction, cell omnipotence can be shown.
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Plant cells have silk division observed
Time of Update: 2021-01-08
animal cells have silk division in the early stages near the nuclear membrane has two central bodies.
。 The main processes in the pre-mid-term are the final formation of the spindle body and the movement of the chromosome towards the equatorial surface.
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Determination of the permeability of plant cells (mass wall separation method)
Time of Update: 2021-01-07
The cell volume in the initial mass wall separation state is slightly smaller than when the absorbent is saturated, so the permeability potential of cell fluid concentration and penetration is slightly lower than that of absorbent saturation.
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Cryopreservation of Plant Cell Suspensions
Time of Update: 2020-12-30
During slow coolingthe extracellular solutions are nucleated and the cells cryodehydrate during controlled cooling as a consequence of extracellularice, to the point where their intracellular fluids will vitrify on subsequent transfer to liquid nitrogen.
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RNAi Expression Vectors in Plant Cells
Time of Update: 2020-11-30
Suppression by double-stranded RNA (dsRNA) of expression of a target gene is known as RNA interference (RNAi).
This methodology should facilitate the analysis of individual gene function in plant cells.
