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Surface Plasmon Resonance Analysis of Interactions Between Replicase Proteins of Tomato Bushy Stunt Virus
Time of Update: 2021-01-18
Understanding the various biochemical activities of the replication proteins can lead to target identification for human intervention to control viral infections or the damage to the host cells.
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PlantVirus Interactions
Time of Update: 2021-01-18
This overview considers these technologies and how they have been used to identify novel viral and plant proteins or genes involved in disease and resistance responses, as well as defense signaling.
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Biochemical Analyses of the Interactions Between Viral Polymerases and RNAs
Time of Update: 2021-01-18
Despite this, only minimal detailed information is available for the interaction between viral polymerases and cognate RNAs. We study the biochemical interactions using two viral polymerases that could serve as models for other plus-strand RNA viruses: the replicase from the tripartite brome mosaic virus (BMV), and the recombinant RNA-dependent RNA polymerase (RdRp) from hepatitis C virus (HCV).
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Studying Interactions Between Chloroplast Proteins in Intact Plant Cells Using Bimolecular
Time of Update: 2021-01-17
Protein–protein interactions play crucial roles in the execution of many cellular functions, including those in plastids.
Identifying and characterising protein–protein interactions can yield valuable information regarding the function of a protein and can also contribute towards understanding protein–protein interaction networks in plastids, thereby contributing to a better understanding of cellular processes.
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Genome-Wide Analysis of RNA-Protein Interactions in Plants
Time of Update: 2021-01-17
To dissect RNA–protein interaction networks, it is necessary to identify the RNAs with which each RBP interacts and to determine how those interactions influence RNA fate and downstream processes.
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Separating the Inseparable: The Metabolomic Analysis of PlantPathogen Interactions
Time of Update: 2021-01-17
Plant–microbe interactions—whether pathogenic or symbiotic—exert major influences on plant physiology and productivity.
We here describe an approach based on co-cultivation ofArabidopsis cell cultures and bacterial plant pathogens to assess the metabolomes of both interacting partners, which we refer to as dual metabolomics.
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Probing Interactions Between Plant Virus Movement Proteins and Nucleic Acids
Time of Update: 2021-01-16
MPs are multifunctional proteins, and one of their functions is almost invariably binding to nucleic acids.
We also describe protocols for purification of recombinant viral MPs over-expressed in bacteria and production of different DNA and RNA probes for these binding assays.
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Blood: The CBL mutation drives the PI3K/AKT signal by enhancing the interaction between LYN and PIX3R1
Time of Update: 2021-01-12
, such as Belizaire, combine functional detection and overall mass spectrometry to define phosphate proteomics, CBL interaction groups, and signal activation mechanisms in a cell line that expresses all-like gene mutations.
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Professors Lu Yuanping and He Qiang, Sichuan University: Interaction between no-eating acid and pig
Time of Update: 2021-01-12
Commsing Author Professor Lu Yuanping Head of The Department of Food Engineering, Secretary of the Party Branch of Sichuan University, Deputy Director of the Key Laboratory of Sichuan University of Food Science and Technology, and Postgraduate Instructor of Food Science, Agricultural Products Processing and Storage Engineering.
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The iPhyClassifier, an Interactive Online Tool for Phytoplasma Classification and Taxonomic Assignment
Time of Update: 2021-01-11
Based on RFLP pattern similarity coefficient scores, thei PhyClassifier gives instant suggestions on group and subgroup classification status of the phytoplasma strains under study.
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Experimental Setups and Considerations to Study Microbial Interactions
Time of Update: 2021-01-06
In this chapter, the use of proteomics for studies of microbial interactions is discussed.
Despite the limitations, proteomics is a useful method, and an important complement to other approaches for studies of microbial interactions.
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Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique
Time of Update: 2021-01-05
The initiation of transcription of eukaryotic genes by RNA polymerases is controlled by complex interactions between nonhistone proteins and specific regulatory DNA sequences (promoters and enhancer
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Preparation of Lipid Membrane Surfaces for Molecular Interaction Studies by Surface Plasmon Resonance Biosensors
Time of Update: 2021-01-05
In this review chapter we describe possible ways to prepare lipid membrane surfaces on various sensor chips and some of the experimental considerations one has to take into account when performing such experiments.
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Fluorescence Resonance Energy Transfer Techniques to Study Ligand-Mediated Interactions of PPARs with Coregulators
Time of Update: 2021-01-04
The fluorescence resonance energy transfer assay is a technique widely used to evaluate coregulator recruitment to nuclear receptors induced by ligands.
With this assay it is possible to quantitatively determine the interaction and the affinity of coregulators with PPARs when these receptors are complexed with ligands.
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Surface Plasmon Resonance Spectroscopy in Determination of the Interactions Between Amyloid Proteins (A) and Lipid Membranes
Time of Update: 2021-01-04
Surface plasmon resonance (SPR) spectroscopy is emerging as a useful tool for determination of molecular interactions in real time.