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J Neurosci—Li Shao/Ma Tonghui's team revealed the orthogonal array structure of AQP4 in aquaporin
Time of Update: 2022-10-02
On September 13, 2022, Professors Li Shao and Ma Tonghui of the School of Basic Medicine of Dalian Medical University published an online publication entitled "AQP4-A25Q Point Mutation in Mice Depolymerizes Orthogonal Arrays of Particles and Decreases Polarized" in the Journal of Neuroscience, an authoritative journal of neuroscience Expression of AQP4 Protein in Astrocytic Endfeet at the Blood-Brain Barrier" research paper.
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biological orthogonal rare earth molecular probes are used for near-infrared fluorescence/mass spectrometry imaging
Time of Update: 2022-09-20
" Spectrometry Imaging" research paper and was selected as a VIP (very important paper) paper 。 The work reported the first bio-orthogonal, high-brightness near-infrared (NIR) rare earth complex molecular probe, which specifically labeled multiple types of biological macromolecules in cells through metabolic labeling strategies and click chemistry reactions, achieved multicolor imaging and click chemistry-based expansion microscopy imaging (Click-ExM), and successfully combined NIR fluorescence imaging and mass spectrometry imaging to achieve bimodal imaging at subcellular resolution.
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Yang Sheng's research group discovers highly active CRISPR transposase for orthogonal multiple editing
Time of Update: 2021-10-01
On September 3, 2021, the international academic journal Nucleic Acids Research published online a research paper entitled "Orthogonal CRISPR-associated transposases for parallel and multiplexed chromosomal integration" completed by the Yang Sheng research group of the Center for Excellence in Molecular Plant Science, Chinese Academy of Sciences .
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probes in the far red region based on "bio-orthogonal engineering"
Time of Update: 2021-04-23
In this work, the two research groups collaborated to use the "inverse electron demand Diels-Alder reaction" to point and specifically label the "proton pump" membrane protein-rhodopsin on the surface of neurons, and then construct a A series of fluorescent membrane potential probes with high sensitivity and imaging signal-to-noise ratio can detect neuronal action potentials in the far red area.
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The transduction of the virus guided by the orthogonal chemical lysis of the sugar metabolism organism enhances the T-cell engineering transformation.
Time of Update: 2020-08-05
Dr. Parijat Bhatnagar, a senior author of the and director of cell medicine at the SRI International Center for Chemical Biology in Menlo Park, California, and his colleagues designed the t-cell "biofactory" to target cell diseases directly in the body while minimizing damage to surrounding healthy cells, the study was published in Advanced Biological Systems.
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Transposons Help Expand the CRISPR Toolbox: Another Highly Efficient Orthogonal CAST System CRISPR RNA-guided insertion of large 10 kb DNA fragments
Time of Update: 2023-01-05
The CRISPR-Cas system in prokaryotes can guide the degradation of exogenous genetic elements such as bacteriophages and plasmids through RNA, enabling DNA-encoding, RNA-mediated, nucleic acid-targete
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BGI Research Reveals the Mutual Orthogonal Molecular Mechanism of Novel PylRS/tRNAPyl
Time of Update: 2022-05-10
On April 25, 2022, a team from Shenzhen BGI Life Sciences Research Institute published online at Nucleic Acids Research (IF=17) entitled "The tRNA discriminator base defines the mutual orthogonality of two distinct pyrrolysyl-tRNA synthetase/tRNA Pyl pairs in the same organism”, which unearthed two novel PylRS/tRNA Pyl pairs that coexist in the halophilic methanogenic archaea Candidatus Methanohalarchaeum thermophilum HMET1 , using the model halophilic archaea H, which is closely related to HMET1.
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Science Sub-Journal: An Orthogonal IL-2/IL-2Rβ System Promotes Persistence of CAR-T Cells and Enhances Tumor Clearance
Time of Update: 2022-04-29
In this preclinical model, administration of STK-009 to mouse recipients receiving hoRb-expressing CAR-T cell transplantation promoted CAR-T cell activation, proliferation, and antitumor efficacy with limited systemic toxic side effects .
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Science Sub-Journal: Human Orthogonal IL-2/IL-2Rβ System Enhances the Proliferation and Antitumor Effects of CAR-T Cells
Time of Update: 2022-04-29
In this preclinical model, administration of ortho-hIL-2 to mouse recipients receiving CAR-T cell transplantation expressing ortho-hIL-2Rβ promoted CAR-T cell activation, proliferation, and antitumor efficacy with Limited systemic toxicity .
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PFGE Using Double-Inhomogeneous Fields or Orthogonal Field-Alternating Gel Electrophoresis (OFAGE)
Time of Update: 2020-12-01
However, later it was recognized that field gradients or inhomogeneous fields were not necessary to separate largeDNA fragments, and nowadays, PFGE is generally used to mean pulsed-field gel electrophoresis, i.e., all kinds of methods involving switching (pulsing) of fields to separate large DNAs. The two configurations discussed here are the original PFGE version with point electrodes and a later version using continuous electrodes ( 2 ) ( see Fig. 1 ).
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Orthogonal method to determine the influence of several factors on enzyme activity
Time of Update: 2020-10-30
1. Bovine serum albumin: 20mL distilled water added bovine serum albumin 2.2g, urea 36g, 1mol/LNaOH solution 8mL, room temperature placed 1h, so that protein denatured.
folin-phenol reagent storage solution makes the acidity eventually 1moL/L before use.
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Orthogonal method to determine the influence of several factors on enzyme activity
Time of Update: 2020-10-28
1. Bovine serum albumin: 20mL distilled water added bovine serum albumin 2.2g, urea 36g, 1mol/LNaOH solution 8mL, room temperature placed 1h, so that protein denatured.
folin-phenol reagent storage solution makes the acidity eventually 1moL/L before use.
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The Application of Orthogonal Chromatography for the Purification of Synthetic Pharmaceutical Intermediates
Time of Update: 2019-02-02
Introduction of application technology research center normal phase chromatography and reverse phase chromatography are two common separation modes of flash preparation chromatography, which are widel