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Identification and Characterization of N-Glycosylated Proteins Using Proteomics
Time of Update: 2020-11-28
Combinations of affinity–enrichment techniques, chemical and biochemical protocols, and advanced mass spectrometry facilitate detailed glycoprotein analysis in proteomics, from fundamental biological studies to biomarker discovery in biomedicine.
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Comparative proteomics using 2-D gel electrophoresis and mass spectrometry as tools to dissect
Time of Update: 2020-11-27
We propose two-dimensional gel electrophoresis (2-DE) and mass spectrometry to define the protein components of regulons and stimulons in bacteria, including those organisms where genome sequencing is still in progress.
The basic 2-DE protocol allows high resolution and reproducibility and enables the direct comparison of hundreds or even thousands of proteins simultaneously.
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Comparative Analysis of Cytoplasmic Membrane Proteomes of Escherichia coli Using 2D Blue Native/SDS-PAGE
Time of Update: 2020-11-23
Two-dimensional blue native (2D BN)/ SDS -PAGE is the method of choice for the global analysis of the subunits of complexes in membrane proteomes.
Immobilization prevents distortion of BN-gel lanes when they are transferred to the 2nd dimension, which lowers variation and greatly improves reproducibility of 2D BN/SDS-gels.
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Shotgun Proteomics of Plant Plasma Membrane and Microdomain Proteins Using Nano-LC-MS/MS
Time of Update: 2020-11-20
Shotgun proteomics allows the comprehensive analysis of proteins extracted from plant cells, subcellular organelles, and membranes.
Previously, two-dimensional gel electrophoresis-based proteomics was used for mass spectrometric analysis of plasma membrane proteins.
The results obtained are easily applicable to label-free protein semiquantification.
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Analysis of Rice Proteins Using SDS-PAGE Shotgun Proteomics
Time of Update: 2020-11-19
In this chapter we describe the workflow used in our laboratory to analyze rice leaf samples using label-free shotgun proteomics based onSDS -PAGE fractionation of proteins.
Rice proteomics has benefitted substantially from successful execution of shotgun proteomics techniques.
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How to Use 2D Gel Electrophoresis in Plant Proteomics
Time of Update: 2020-11-15
Nevertheless, 2D gel-based proteomics still has valuable features, and sometimes unique ones, which make it often an attractive choice when a proteomics strategy must be selected.
These features are detailed in this chapter, as is the rationale for selecting or not 2D gel-based proteomics as a proteomic strategy.
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Analysis of Meiotic Protein Complexes from Arabidopsis and Brassica Using Affinity-Based Proteomics
Time of Update: 2020-11-11
Here we describe the preparation of meiotic protein complexes fromArabidopsis thaliana and its close crop relative,Brassica oleracea , by co-immunoprecipitation for in-solution analysis by tandem mass spectrometry (MS/MS).
thaliana and providing evidence of anin planta interaction between BoASY1 and BoASY3 inB.
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Global Quantitative Proteomics Using Spectral Counting: An Inexpensive Experimental and Bioinformatics Workflow for Deep Proteome Coverage
Time of Update: 2020-11-10
There have been many recent reviews on quantitative proteomic approaches, discussing the strengths and limitations of each.
Unfortunately, there are few detailed methodological descriptions of any one of these quantitative approaches.
Here we present a detailed bioinformatics workflow for one of the simplest, most pervasive quantitative approach—spectral counting.
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, used in situ bacterial transcription and proteomics in plants to identify pathogen proteins attacked by the immune system!
Time of Update: 2020-07-21
Professor, School of plant science and technology, State Key Laboratory of agricultural microbiology, Huazhong Agricultural University, and independent PI Kenichi Tsuda of Mapu Plant Breeding Research Institute are the corresponding authors of the paper.
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Yu Xiaobo/Li Yongzhe team used SARS-CoV-2 full proteomic chip to fully reveal the expression
Time of Update: 2020-07-21
The Proteome-wide analysis of differentially-expressed SARS-CoV-2 antibodies in in [2] was used to detect and analyze the expression and distribution of new crown virus antibodies in the serum of patients with early new coronavirus pneumonia and suspected patients (Fig. 1).
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Proteomics market will reach 17.2 billion US dollars in 2017
Time of Update: 2013-09-06
According to the new market research report released by markets and markets, the global proteomics market will increase to US $17.2 billion in 2017 Despite the current poor economic climate, the CAGR