A new method of sensitive detection of intermediate products produced during the transposing of reverse transcription transposons in crops.

On December 11, 2018, Nature Plants published online the Center for Excellence in Molecular Plant Sciences/Institute of Plant Physiology and Ecology of the Chinese Academy of Sciences, which is part of the Center for Excellence for Plant and Stock Sciences of the Chinese Academy of Sciences. CEPAMS) JungnamCho Research Group, entitled Sensitivedetection of the pre-integration intermediates of the LTR retrotransposons in crops, proposes a new method for sensitive detection of intermediate products produced during the transludes transfer process in crops, such as rice.
reverse transcription transners play an important role in the evolution of the host genome.
researchers largely speculate on their importance from the DNA sequence information they retain edimen in evolution, but such studies can only speculate on events that occurred during the historical evolution of transposons, but they cannot predict their current transposing potential.
has previously suggested a technique called Sequence-Independent Retrotransposon Trapping (SIRT) to determine the presence of extrachromosomal linear DNA (extrachromosomal linear DNA, eclDNA) to indicate the presence of an active LTR reverse transcription transformer in the amoeba.
However, SIRT cannot be used in a genome as large as rice, which is rich in transposons.
the study suggested a new method called aoarfor LTR of eclDNAs bydd (ALE-seq) to compensate for the lack of SIRT.
ALE-seq sequences the end of the LTR 5' after in vitro transcription and reverse recording.
using this method, the study detected the eclDNA of a LTR inversion transposon Go-on that belongs to the Copia family and is activated at high temperatures.
at the same time, researchers sequencing different rice materials found that Go-on swelled in indica at high temperatures.
, ALE-seq was used in tomatoes to identify the reverse transcription transctors of the Gypsy family that regulated the fruit's development.
this is a transport ingenuity for alE-seq analysis, which can be used directly to discover new active reverse transcription transludes without the need for reference comment information.
, therefore, this approach facilitates the assessment of LTR reverse transcription transposonal activity in organisms that do not have reference genomic information or that have low reference genomic information quality.
Source: Institute of Plant Physiology and Ecology.