AGING CELL: Long-term dietary restrictions make hematopoietic stem cells younger.

Hematoblast transplantation (HCT) is the key method to treat a variety of hematostomas.
with the world's ageing population significantly expanding, the number of senior HCT recipients and donors increased.
, the risk of total mortality after HCT increases by 5.5 per cent for every 10 years after the donor ages 60.
study found that older HSCs showed regenerative decline, especially in lymphatic regeneration.
therefore, exploring ways to improve the re-creation capabilities of HSCs from elderly feeds may be of considerable significance to HCT.
, researchers have found that early dietary restriction (DR) can improve the regeneration of HSC in early aging C57BL/6 J mice.
, however, the effects of mid-term DR on the selection of HSCs and un purified hematocytes, which are mainly used in clinical HCT, have not been reported.
Recently, a team of researchers from Nanchang University's Second Affiliated Hospital, published in the journal Age Cell, analyzed the effects of short-term (4 months) and long-term (9 months) treatment of C57BL/6 J mice on the function of HSCs and bone marrow cells in transplantation and undisturbed conditions, starting at 15-18 months of age.
15-18 months of age were about 50-60 years old in humans.
researchers found that short-term DR significantly reduced the size of the HSC library in older mice, particularly bone marrow-type HSCs (CD150highHSCs and CD41-HSCs), while lymphatic HSCs (CD150lowHSCs and CD41-HSCs) did not change.
addition, DR significantly saved the aging-related changes in the LT-HSCs ratio, but failed to save the decline of LMPPs.
to study the effects of DR on HSC function, the purified HSCs in the treated mice were transplanted with competitor cells.
interestingly, DR-derived HSCs resulted in higher inlays of whole white blood cells (WBC) and B lymphocytes, higher inlays of B cells/marrow cells, and similar inlays in bone marrow cells.
, however, clinical HCT mainly uses unrealized hematocytes as a source of transplantation.
short-term DR improves the regeneration of HSCs themselves, it also reduces the frequency of HSCs, which may have a negative impact on HCT.
note that short-term DR showed neutral effects in bone marrow transplantation (BMT), indicating that the functional improvement achieved by short-term DR did not compensate for the decrease in HSCs frequency.
this, the researchers further conducted another competitive BMT, using more bone marrow cells obtained from DR donor to achieve the same number of HSCs transplants as AL donor.
, the bone marrow cells of DR donor produced higher lymphocyte and bone marrow cell chimlines in competitive continuous transplantation.
further studied the effects of long-term (9-month) DR on older mice.
effects of long-term DR on HSCs and ancestral cells were similar to those of short-term DR, cd150lowHSCs were significantly reduced.
intriguingly, long-term DR increases the chimline of B cells and bone marrow cells in competitive BMT.
addition, DR donor produces a higher proportion of chimlines in B-cells/bone marrow cells.
it is important to note that the beneficial effects on lymphocytes begin as early as 1 month after BMT, which is essential for early infection resistance after transplantation.
the mechanism, long-term DR revitalizes abnormally regulated mitochondrial pathlines in aging HSCs, accompanied by an increase in HSCs during rest and a decrease in DNA damage signals.
-term DR has a similar trend in saving these signs of aging, but to a much lesser extent.
, current studies have shown that medium-term DR can improve the function of aging HSCs, and long-term DR can even improve hemagtic reconstruction in bone marrow transplants, which may have considerable implications for human HCT in the case of only elderly providers.
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