Blood: Plasma particles affect endothelial cells differently when patients with sickle cell disease have different effects on endothelial cells

Particles (MPs) are submicron extracellular vesicles that can be detected in large quantities in the blood circulation of patients with sickle cell anemia (SS)Several studies have studied the biological effects of MPs in vitroGarnier et alanalyzed for the first time the effects of circulating MPs on endothelial cells (ECs) in 60 patients with sickle cell disease (SCD)SCD MPs were collected and compared with MPs isolated from healthy individuals, in addition to plasma MPs in SS patients with hydroxyurea (HU) and 2 years after treatment, as well as plasma MPs in SS patients with vascular obstruction and stable stateSS MPs increase mRNA and protein levels of endothelial cell ICAM-1 and neutrophil adhesion compared to AA MPsICAM1 overexpression is mainly caused by MPs derived from red blood cells, MPs derived from platelets have no such induction effect;had a reduced ability to induce endothelial cells to promote inflammatory manifestations in SS patients after HU treatment compared to MPs collected before treatmentConversely, MPs released during vascular obstruction can increase ICAM-1 and neutrophil adhesion levels in a PS-dependent manner compared to MPs collected in a stable stateIn addition, closed anti-ICAM-1 antibodies can eliminate neutrophil adhesion, this study proves that MPs play an important role in SCH pathophysiology by triggering the pro-inflammatory phenotypes of endothelial cells, revealing a new model of HU's role, and suggesting potential new therapies, namely membrane-linked protein V and anti-ICAM-1 antibodies