Blood: Regulation mechanism downstream of platelet collagen receptor GPVI

!---- platelets are involved in the healing of vascular damage through coordinated adhesion, secretion, and aggregation reactionsrapid and progressive changes in platelet morphology patterns and functions are coordinated downstream by specific receptors on platelet surfaces, and the intracellular signaling mechanism that mediates the process is not clearthe study, the cell physiology and phosphorylation proteomics methods combined with peptide series mass label (TMT) markers, sample multiplexing, synchronous precursor selection (SPS) and three-stage tandem mass spectrometry (MS3) to analyze the signal mechanism downstream of immunotyrosine activated mold (ITAM) platelet collagen GPVIoverall, more than 3,000 significant phosphorylation events (FDR-lt;0.05) were detected on more than 1,300 proteins under the initial and progressive conditions of PLATElet activation mediated by GPVI, through a literature-directed causal inference tool, the researchers matched more than 300 site-specific signaling pathway relationships from phosphorylation proteomic data from key, emerging GPVI effect factors such as FcRg, Syk, PLCg2, PKCd, DAPP1in the context of relevant knowledge, the regulatory network of GPVI/ITAM signals has also been clarified, including more than 40 Rab GTPases and related regulatory protein systems, of which TAK1-mediated Rab7 S72 phosphorylation is related to the maturation of the nesolysussomease and the function of the PEVI-mediated plateletsin addition toas a model for generating and testing assumptions from histological data sets, this study proposes a method to identify hemostatic effects, biomarkers, and therapeutic targets associated with the state of thrombosis, vascular inflammation, and other platelet-related diseases