Blood: Single-cell sequencing reveals transcription factors and molecular pathways that drive HSC in vitro production

!---- hematopoietic stem cells and progenitor cells (HSPc) develop through different waves at different anatomical sites during embryonic developmentin vitro differentiation of human pluripotent stem cells (hPSC) can reproduce some of these processes, but functional hematopoietic stem cells (HSCs) have proved difficult to produceTo analyze the dynamics and heterogeneity of HPSC in vitro production, the researchers used single-cellRNA sequencing (scRNAseq) to combine single-cell protein expression analysisbioinformatics analysis and functional validation identified the transcription groups of infant progenitor cells and red blood cells, as well as macronuclear and white blood cell stereotyped progenitor cells, and identified CD44, CD326, ICAM2/CD9, and CD18 as the markers of these progenitor cellsusing an artificial neural network (ANN) based on scRNAseq in the human fetus's liver, the researchers were able to identify a variety of hPSCs derived HPSC phenotypes, including a small class classified as HSCsthis transient HSC-like cell population decreases with the progression of differentiation, and eventually disappears completely in the data set of cells screened based on CD43 expressionby comparing the single-cell transcription group of in vitro-produced HSC-derived cells with the single-cell transcription group produced in the fetal liver, the researchers identified transcription factors and molecular pathways that could be used in the future to improve the in vitro production of HSC