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    Home > Active Ingredient News > Blood System > Br J Haematol: New discovery can lift the veil of athletes using blood doping

    Br J Haematol: New discovery can lift the veil of athletes using blood doping

    • Last Update: 2021-07-31
    • Source: Internet
    • Author: User
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    Red blood cell (RBC) transfusion is the most common treatment for hospitalized patients
    .
    The red blood cell unit can be refrigerated for up to 42 days and undergo various biochemical and morphological changes (storage damage) during storage

    .
    However, the effect of storage on the transcriptome of red blood cells has not been well studied.
    Regarding whether athletes use blood doping in competitions, although one of the three most common methods to greatly improve the blood oxygen carrying capacity of athletes has been developed There are two types, but the so-called "autologous" or self-transfusion has been unable to detect

    .

    Recently published in the of Haemotology British Journal magazine , a Duke University research team has found a way to help sports officials to detect whether or not the athlete's blood was injected into their own stored blood
    .

    Haemotology British Journal of British Journal of Haemotology magazine magazine

    Autologous blood transfusion is to take part of the athlete's blood before the competition, and only select the red blood cells, and then transfer these cells back to the athlete before the competition to improve the blood's ability to carry oxygen, and oxygen is an important fuel for muscle performance
    .
    The best test method used by the World Anti-Doping Agency (WADA) so far is the "athlete passport", which compares blood samples taken before the game with blood samples taken during the game to see if there are "significant" changes in biochemistry

    .

    Researchers study nucleic acids in red blood cells, especially RNA
    .
    It has long been believed that red blood cells lack nucleic acid because they do not carry the nucleus, and DNA is usually found in the nucleus

    .
    Although it was once considered to lack nucleic acid, rbc actually contains a rich variety of RNA species

    .
    Among these RNAs, there are short RNA fragments called microRNAs (miRNA), which are commonly used to control the production of proteins in cells

    .

    The researchers took three units of blood from the volunteers and processed them to remove almost all white blood cells and approximately 80% of the plasma, leaving a relatively pure sample of red blood cells, just as required for autologous blood transfusion Like that
    .
    The RNA samples extracted from these cells were extracted and analyzed at intervals ranging from 1 day to 42 days

    .
    When comparing the subsequent samples with the samples on day 1, the RNA changes related to storage become obvious

    .
    Two types of miRNAs increase in number during storage, and two types decrease

    .
    One form of increase, called miR-720, has the most significant and consistent changes

    .

    Increased expression of "miR-720" during red blood cell storage

    Increased expression of "miR-720" during red blood cell storage

    Adding recombinant angiotensin to the stored rbc can further enhance the induction of "miR-720"
    .
    On the contrary, the
    immunodepletion method to remove angiotensin in the red blood cell lysate can significantly reduce the lysis rate of tRNA
    .
    These data indicate that Ang in rbcs contributes to tRNA

    .
    Enzymes cleave "miR-720" during red blood cell storage
    .

    Vascular immune enzymes

    Angiotensin ( of angiogenin in ) promotes the storage of RBCs in miR-720 increased

    Angiotensin ( Angiogenin ) promotes the storage of RBCs in miR-720 increases Angiogenin promotes stored RBCs in miR-720 increased

    These data indicate that red blood cell storage is associated with significant and reproducible "miR-720" induction
    .
    The increase of "miR-720" is one of the reasons for the increase of miRNAs in stored red blood cells

    .
    This is the first study that emphasizes the dramatic increase of "miR-720" and uses biochemical properties to explain the underlying mechanism of Ang

    .
    This reproducible and novel characteristic of stored red blood cells can be used as a biomarker for monitoring stored red blood cells

    .

    In the future, it will be of great interest to determine any functional role of highly expressed miR-720 in stored red blood cells as well as endothelial cells and macrophages
    .
    Another possible application is the investigation of blood stimulants

    .
    Although athletes’ autologous blood transfusions are difficult to identify with routine tests, according to the presence of red blood cells, red blood cells whose levels of miR-720 are significantly higher than those of normal circulating cells can be detected

    .
    Further research is needed to determine the signal that stimulates angiotensin activation during red blood cell storage, as well as the role of activation of angiotensin, continuous RNA processing, and/or miR-720 induction in the development of red blood cell aging and red blood cell storage damage

    .

    Article reference: Wen-Hsuan Yang, Jennifer Doss, Katelyn Walzer, Shannon McNulty, Jianli Wu, John Roback and Jen-Tsan Chi.
     
    Angiogenin-mediated tRNA Cleavage as a Novel Feature of Stored Red Blood Cells .
     
    British Journal of Haemotology , Oct.
    18, 2018 DOI: 
    10.
    1111/bjh.
    15605

    Article reference: Wen-Hsuan Yang, Jennifer Doss, Katelyn Walzer, Shannon McNulty, Jianli Wu, John Roback and Jen-Tsan Chi.
     
    Angiogenin-mediated tRNA Cleavage as a Novel Feature of Stored Red Blood Cells Angiogenin-mediated tRNA Cleavage as a Novel Feature of Stored Red Blood Cells .
     
    British Journal of Haemotology British Journal of Haemotology , Oct.
    18, 2018 DOI: 
    10.
    1111/bjh.
    15605
    10.
    1111/bjh.
    15605
    10.
    1111/bjh.
    15605

     



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