Check out your PCR/qPCR Sealing Membrane Selection Guide!

introduction

PCR (polymerase chain reaction), as one of the routine and necessary experimental techniques for biomolecular experiments, has been widely used

PCR/qPCR sealing film background

According to the principle of PCR detection signal, it can be divided into ordinary PCR and quantitative PCR (qPCR)

As far as single-tube and eight-tube tubes are concerned, whether it is PCR for end-point detection or qPCR for real-time detection, we need to choose products with good thermal conductivity and uniformity of tubes, and good sealing of tube caps and tube bodies

PCR/qPCR Sealing Membrane Selection Guide

Sometimes it is easy to confuse the sealing membranes of PCR and qPCR, resulting in poor qPCR results, waste of samples, time and cost

As far as ordinary sealing membranes are concerned, the material is mostly polypropylene, free of RNase/DNase and nucleic acid.

Compared with ordinary PCR sealing film, since the optical path detection channels of many qPCR instruments are located at the top, this makes it more demanding on the transparency of the sealing film; secondly, it requires an autofluorescence background; in addition, the evaporation rate is low and airtight.

Therefore, when looking for PCR or qPCR sealing membranes, you need to choose based on the above factors

Introduction of Plateseal Sealing Film

Plateseal has more than 20 years of expertise in membrane design and manufacture, and offers a wide variety of high-quality, high-performance PCR sealing membranes

# Transparent polyester film

• Suitable for all kinds of PCR plates

# Pressure sensitive sealing film

• Low autofluorescence, improve signal-to-noise ratio

 # Professional qPCR sealing film

• Polyester material, light transmittance up to 86 %

 # Professional qPCR sealing film (large area)

• Polyester material, transmittance up to 86 %

If you are interested or know more about Plateseal sealing film, you can directly scan to contact our product specialist!

As Plateseal's partner and national agent in China, Gene Co.