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    Home > Food News > Enzyme News > Convenient SNP typing detection reagent [new product recommendation]

    Convenient SNP typing detection reagent [new product recommendation]

    • Last Update: 2021-08-10
    • Source: Internet
    • Author: User
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    In addition to the qualitative/quantitative detection of infectious pathogens, the real-time fluorescent PCR system can also be applied to the study of genetic variation


    Allelic single nucleotide polymorphism (SNP) and structural variation are the basis of genetic variation in individuals and populations


    The LightMix® kit uses a hybridization probe combined with a melting curve analysis genotyping method, which can complete qPCR amplification and typing detection in the same reaction tube at one time.


    LightMix® kit

    MTHFR C677T Gene Polymorphism Detection Kit

    5,10-Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in human DNA synthesis and homocysteine ​​metabolism.


    The current international and domestic research results show that [1], the enzyme activity of heterozygous mutant MTHFR677CT decreased by 30%, and the activity of homozygous 677TT enzyme decreased by more than 65%


    Principles of kit detection:

    A specific primer pair was used to amplify the 233bp fragment on MTHFR.


    Melting curve analysis used to be used to determine the specificity of the amplified product after the SYBR Green detection reaction.



    Run the conventional qPCR reaction program to carry out the amplification of the target fragment and the fluorescence signal detection.


    ◀ LightMix® Kits are used for SNP typing detection kits using hybrid probes or simple probes to obtain genotyping information through melting curve analysis


    LightMix® kit typing test advantages

    • Realize high-throughput and automated typing detection on the qPCR platform


    Multiple SNP typing test reagents for the study of genetic variation related to cardiovascular disease

    Test items

    Related genes

    product number

    MTHFR C677T

    Methylenetetrahydrofolate reductase gene C677T

    0 6896367001

    MTHFR A1298C

    Methylenetetrahydrofolate reductase gene A1298C

    0 6896383001

    PAI-1 4G/5G

    Plasminogen activator inhibitor -1 gene 4G/5G

    06896359001

    IL6 G174C

    Interleukin 6 gene G174C

    05945585001

    Factor XIII A V34L

    Coagulation factor XIII A gene V34L

    0 6896405001

    ApoB -100 R3500Q

    Apolipoprotein B -100 R3500Q

    0 7029357001

    ApoE C112R R158C

    Apolipoprotein E C112R R158C

    0 6703500001

    CYP2C9

    Cytochrome 2C9*2,*3

    0 5945712001

    VKORC1C1173T/G1639A

    Vitamin K epoxide reductase

    0 5947154001

    Research on Drug Metabolism and Other Genetic Variations

    Test items

    Related genes

    product number

    CYP2C9

    Cytochrome 2C9*2,*3

    0 5945712001

    CYP2C19

    Cytochrome 2C19*2,*3

    0 5945739001

    VKORC1C1173T/G-1639A

    Vitamin K epoxide reductase

    0 5947154001

    CYP 2D6

    Cytochrome 2D6*3, *4, *5/*5

    0 6296742001

    ACE I/D

    Angiotensin converting enzyme

    0 7161182001

    AAT Pi*S Pi*Z

    α-I type antitrypsin

    0 7055684001

    Factor II G20210A

    F2 , hemagglutinin

    0 6896502001

    Factor V (

    Leiden

    )

    Factor V Leiden mutation

    0 6896529001

    FGB -455G/A

    Human fibrinogen ß gene

    0 163142001

    HLA-B27

    HLA-B27 gene

    0 6896537001

    eNOS E298D

    Endothelial nitric oxide synthase gene

    0 7023766001

    IL23R Arg381Gln

    Interleukin 23 receptor gene

    0 5967287001

    IL6 G-174C

    Interleukin 6 gene G-174C

    0 5945585001

    Description:


    Request detailed information of LightMix qPCR test kit now

    The product is for life science research only, not for diagnostic procedures.


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