Diabetologia: The absence of h-iron protein in macrophages can reduce obesity and diabetes caused by a high-fat diet in mice

Iron protein is a protein that stores iron and plays a vital role in iron metabolism.
iron accumulation affects obesity and diabetes, both of which can be improved by reducing iron.
ferrine reduces toxicity through its ferrooxidase activity, thus acting as a cell protection.
researchers studied the role of macrophage h-iron protein in obesity and diabetes.
use condition macrophage-specific h-iron protein (Fth, also known as Fth1) knock-out (LysM-Cre Fth KO) mice, divided into normal diet (ND) wild type (WT) and LysM-Cre Fth KO mice, high-fat diet (HFD) wild type (WT) and LysM-Cre Fth KO mice.
analyzed obesity and diabetes characteristics, tissue iron content, inflammation, oxidative stress, insulin sensitivity, and metabolic measurements in the mice.
in-body experiments using RAW264.7 macrophages.
the iron concentration of macrophages decreased in LysM-Cre Fth KO mice, and the expression of ferrin mRNA increased.
HFD-induced obesity low LysM-Cre Fth KO mice at 12 weeks (weight: KO 34.6±5.6 g vs WT 40.1± (5.2 g), messenger rna expresses inflammatory cytokines and penetrates adipose tissue macrophages and oxidative stress increases in HFD-fed WT mice, but does not raise HFD-fed LysM-Cre Fth KO mice.
, however, increased iron concentrations in adipose tissue and spleen in wild mice fed with HFD, while LysM-Cre Fth KO mice fed with HFD (adipose tissue (white moth iron/g protein)) did not observe this phenomenon (KO 1496±479 vs WT 2316±866; Spleen (mol Fe/g protein): KO 218±54 vs WT 334±83).
addition, HFD impaired both glucose tolerance and insulin sensitivity in WT mice, but improved in LysM-Cre Fth KO mice.
energy consumption, mRNA expression and body temperature of heat-producing genes were all higher in KO mice at high temperature fd than in WT mice at high temperature fd.
in-body experiments showed that iron content decreased in the macrophage line of transfth siRNA, and the increase of Tnf-a (Tnf) mRNA induced by polysaccharine was inhibited.
the absence of macrophage h-iron protein inhibits inflammatory responses by reducing intracellular iron levels, thereby preventing obesity and diabetes caused by hand, foot and mouth disease.
the results of this study highlight macrophage iron levels as a potential therapeutic target for obesity and diabetes.
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