Digital PCR technology for precise detection of viral RNA mutations [Innovative Tips]

Bio-Tong reported that researchers in the United States and Canada found that Bio-Rad’s droplet digital PCR (ddPCR) technology can achieve higher accuracy, sensitivity, and accuracy compared with traditional reverse transcription quantitative PCR (RT-qPCR) technology.

This is the first article that directly compares ddPCR technology and RT-qPCR technology

Many conditions will cause the immune system to decline, making the body more susceptible to virus infection

In this study, scientists at Bio-Rad and collaborators at Laval University in Canada used a mixture of mutant and wild RNA, which came from 2009 flu patients

Since droplet digital PCR is based on absolute quantification, while RT-qPCR relies on relative quantification, ddPCR avoids the inherent changes of RT-qPCR

Guy Boivin, the chief scientist of Canadian flu and other respiratory viruses, led the research

Researchers believe that influenza viruses are just one of many viruses that benefit from early detection

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Original Search

Optimization of Droplet Digital PCR from RNA and DNA extracts with direct comparison to RT-qPCR: Clinical implications for quantification of Oseltamivir-resistant subpopulations

Journal of Virological Methods