Experimental design One of the Drop-off digital PCR methods: detection of gene mutations and gene deletions

The main advantage of the drop-off digital PCR detection method is that it can detect homologous gene mutations (including nucleotide deletions, insertions and substitutions) with a short genomic sequence in one reaction

The information in Figure 1 is described as follows:

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The drop off calculation method can be obtained in the following ways:

Loginor Whale, AS, et al, Biomol Derect Quantif 2016 27:.

Drop-off detection of KRAS, NRAS and EGFR hotspot mutations

In clinical applications, a set of predictive genetic markers are usually tested to track the effect of treatment

The information in Figure 2 is described as follows:

The 7 and 4 most common mutations in KRAS exon 12 and NRAS exon 3, and the reliability of the Drop-off test for the deletion of EGFR exon 19 were detected respectively

The information in Figure 3 is described as follows:

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Since there are many mutations in KRAS and NRAS mutation hotspots, and there are deletions or insertions of different lengths in exon 19 of EGFR, the drop-off experimental design has achieved rapid, economical, and effective use of the fewest kinds of probes.

Application highlights:

Drop-off digital PCR detection can simultaneously detect multiple mutations in hot spots of the genome.

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