Experts from the Academy of Agricultural Sciences use RPA to quickly detect genetically modified crops

Biocom reports: Recombinase polymerase amplification of RPA is known as a nucleic acid detection technology that can replace PCR .

Since the first commercialization of genetically modified crops, nearly two decades have passed, and the development of this technology has always been accompanied by fierce debate

A few days ago, the research team of the Institute of Biotechnology of the Chinese Academy of Agricultural Sciences realized the rapid detection of genetically modified crops on the basis of RPA .

The promoter of cauliflower mosaic virus 35S ( CaMV-35S ) and the terminator of the nopaline synthase gene ( nos ) of

RPA can quickly detect trace amounts of DNA in samples at room temperature ( 37–42 °C ) .

Studies have shown that RPA can greatly simplify the reaction process, significantly shorten the detection time, and is an effective way to quickly detect genetically modified crops

About the Author:

Wan Yu Song Bo Shi associate professor, master tutor

Mainly engaged in research on the environment and food safety of genetically modified organisms and their products, presided over and participated in major genetically modified special projects related to genetically modified organisms safety research, the national 973 plan, 863 plan, the Tenth Five-Year

Obtained a doctorate degree in crop genetics and breeding from the Graduate School of the Chinese Academy of Agricultural Sciences in 2002 ; engaged in post-doctoral research at the University of Tokyo in Japan from 2003 to 2005 ; engaged in research on the safety evaluation and testing of genetically modified organisms at the Institute of Biotechnology, Chinese Academy of Agricultural Sciences from 2006 to present

Biological Communication Editor: Ye Yu

Recommended original text by Biology:

Recombinase Polymerase Amplification (RPA) of CaMV-35S Promoter and nos Terminator for Rapid Detection of Genetically Modified Crops