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Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis

 Last Update: 2021-01-31
 Source: Internet
 Author: User

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plasmid transfection

essential genes

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Plasmid vector construction is an essential step for molecular microbiology yet often a time-consuming process. Manipulation of the fungal genome to express genes to activate secondary metabolite production often requires creation of plasmid constructs in a reiterative fashion. Here we introduce a modified Quick-change site-directed mutagenesis method that allows for rapid and accurate construction of fungal transformation vectors.

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