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Background: Diabetic retinopathy (DR) is the most common microvascular complication caused by diabetes and has become one of the most important causes of blindness
Diabetic Retinopathy (DR) Diabetic Retinopathy (DR) Yin Deficiency Fire Prosperity Blood Stasis Water Stop
Simiao Yong'an Decoction (SMYAD) from the Qing Dynasty "Prescription New Edition" has the effects of clearing away heat and detoxification, promoting blood circulation and relieving pain , and is traditionally used to treat sores, carbuncles and ulcers
Simiao Yong'an Decoction (SMYAD) Simiao Yong'an Decoction (SMYAD) clears heat and detoxifies, activates blood and relieves pain in the treatment of peripheral vascular disease
OBJECTIVE: To explore the protective effect of SMYAD on DR by combining network pharmacology and in vivo experimental evidence, and to further reveal its mechanism of action
Methods: 45 male C57BL/6 mice were intraperitoneally injected with streptozotocin (STZ, 55 mg/kg) to induce diabetes, and 15 mice in the control group were injected with normal saline
The blood-retinal barrier (BRB) dysfunction was observed by Evan's blue leakage test, detection of tight junction (TJ) protein expression, and counting the number of capillaries in retinal detached cells
Evan's blue leak test, detection of tight junction (TJ) protein expression, count of retinal detached cell capillaries
The possible pathways were screened by network pharmacology analysis, and verified by immunofluorescence staining, qPCR, Western Blot, and ELISA
Network pharmacology immunofluorescence staining, qPCR, Western Blot, ELISA
Results: Elevated Evan's blue dye leakage was reduced in the retina of diabetic mice after treatment, the expression of retinal claudin1 and claudin19 (two typical tight junction proteins) was decreased and enhanced, and decellularized capillaries marked by black arrows in the retina The number of blood vessels was reduced, suggesting that SMYAD alleviated BRB dysfunction in vivo
SMYAD alleviates BRB dysfunction in vivo
Regulates inflammation, immune response and angiogenesis Regulates inflammation, immune response and angiogenesis Tumor necrosis factor (TNF) Hypoxia-inducible factor 1 (HIF1) inhibits activation of retinal NF-κB and HIF1α
Conclusions: This study is the first to demonstrate in vivo the alleviating effect of SMYAD on DR
First in vivo demonstration of SMYAD-relieving effect on DR
The disadvantage is that the current network pharmacology database cannot contain all the signaling molecules involved in the development of DR, and there may be other important molecules that have not been found in this study and need to be further explored in the future
Source:
Du A, Xie Y, Ouyang H, et al.
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