Gastroenterology: The occurrence of prostaglandin E2 stimulating ultra-sensitizing reactions in mice in the colon tissue of patients with irritable bowel syndrome

Background and targetvisceral hypersensitivity is common in patients withirritable bowel syndrome(IBS)This study investigated whether inflammatory molecules (such as histamines and proteases) activated peroxidase 2 (COX2) to increase the synthesis of hypertrophic cells into prostaglandins e2(PGE2), thereby activating prostaglandins (EP2)Method
researchers used an enzyme-linkedimmuneadsorption test to determine the molecules in the patients with diarrhea IBS (IBS-D; 18 women and 5 men; ages 28-60), healthy individuals (control group n s 24) in colon mucosa fat cells The level sedituated spontaneously, while the researchers administered intra-colon drugs to mice and rats, cleansed the colon biopsies, histamines, PGE2, small interfering RNA or F2R agonising for EP2, and finally measured the visceral movement of rodents on colon expansionresultshad higher PGE2 levels and COX2 messenger RNA and protein levels in IBS-D patients based on enzyme-linked immunosorption compared to control group biopsy tissueImmune tissue chemistry shows that most COX2 is located in large cellsColon perfusion in rats with IBS-D biopsy of liquid can increase the visceral movement response to colon expansion by three to four times This is associated with a significant increase in PGE2, histamines and trypsin in the colon mucous membrane Inhibition of hypertrophic cell stabilizers, COX2 inhibitors or EP2 can prevent these increases The biopsy of patients injected with IBS-D in the colon failed to induce an internal hypersensitivity response conclusions colon hypertrophy cells abnormalsynthesis of PGE2 appears to induce visceral hypersensitivity in patients with IBS-D