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    Home > Active Ingredient News > Digestive System Information > Gut: Reprogramming the spleen into a functioning 'liver' directly in the body without organ transplantation

    Gut: Reprogramming the spleen into a functioning 'liver' directly in the body without organ transplantation

    • Last Update: 2022-01-22
    • Source: Internet
    • Author: User
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    The liver (liver) is an important metabolic organ in the body of vertebrates, and fully exerts key functions such as detoxification, deoxidation, storage of hepatic glycogen, and synthesis of secreted proteins in the body


    liver

    In modern society, the phenomena of staying up late, insomnia, anxiety, drinking and eating irregularly are more and more common, and more and more people suffer from liver disease, among which liver cancer is even more disgusting


    Orthotopic liver transplantation is the most effective treatment for end-stage liver disease


    On January 7, 2022, the team of Professor Dong Lei from the School of Life Sciences of Nanjing University and the State Key Laboratory of Medical Biotechnology published an original research paper online on Gut titled: Reprogramming the Spleen into a Functioning "Liver" in vivo


    On January 7, 2022, Professor Dong Lei's team from the School of Life Sciences of Nanjing University and the State Key Laboratory of Medical Biotechnology published an original research paper online on Gut entitled: Reprogramming the Spleen into a Functioning "Liver" in vivo

    In this study, material-induced tissue remodeling and direct reprogramming techniques were used to reprogram spleen fibroblasts into hepatocytes in situ, which truly allows the spleen to grow its own liver tissue and realize the functional transformation of the spleen to the liver


    Prof.


    In June 2020, Dong Lei, Zhang Junfeng and Wang Chunming published a paper in the journal Science Advances, transforming the spleen into an organ with typical liver functions in live mice


    They injected a preselected tissue extract into the spleen of mice that showed a lower immune response and produced more of the extracellular matrix needed for cell growth


    Professor Dong Lei told Biology World that the team's research published in Science Advances is to transplant liver cells into the spleen, which solves the problems of growth, integration and functionalization of the regenerated liver, but has not yet solved the problem of immune rejection and the source of seed cells for these tissues The inherent problem of regeneration


    In this latest study, the research team firstly moved the spleen from the abdominal cavity to the subcutaneous area to facilitate continuous injection and observation of the spleen, and then injected SiO2 nanoparticles into the spleen to promote the proliferation of fibroblasts in the spleen for subsequent reprogramming and liver regeneration


    Next, the research team used lentivirus as a delivery vehicle to overexpress liver-specific transcription factors (Foxa3, Gata4, and Hnf1a) in spleen cells, allowing splenic fibroblasts to transdifferentiate into functional hepatocytes in situ


    Finally, the proliferation of transdifferentiated hepatocytes in the spleen was further promoted by delivering tumor necrosis factor-α (TNF-α) and lentivirus overexpressing epidermal growth factor (EGF) and hepatocyte growth factor (HGF) to the spleen


    More importantly, in the experiment of acute liver failure induced by 90% hepatectomy, the survival rate of mice that successfully constructed transformed spleens was 50%, while all the untransformed mice died, indicating that transformed spleens can compensate for liver function.


    In the experiment of acute liver failure induced by 90% hepatectomy, the survival rate of mice that successfully constructed transformed spleen was 50%, while all the untransformed mice died, indicating that transformed spleen can compensate for liver function

    This study is different from the existing liver regeneration methods such as whole organ transplantation, tissue engineering or cell transplantation.


    The problems of donor cell shortage and immune rejection faced by cell transplantation provide a new solution for the functional reconstruction of large tissues and the management of end-stage liver diseases

    Finally, Professor Dong Lei said that the team has developed a series of biological and material technologies around spleen matrix modification and in situ tissue regeneration, and carried out a variety of methods and tissue exploration.


    Original source:

    Original source:

    Chunyan Liu, et al.


    Reprogramming the spleen into a functioning 'liver' in vivoLeave

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