HAEMOPHILIA: Observation of pathological changes and expression of ligaments in patients with hemophilic arthritis

Hemophilic arthritis (HA) is the most common and most serious complication in patients with hemophilia, which can lead to advanced joint dysfunction and disability
.
Previous studies on the pathological mechanism of HA focused on synovial bone, cartilaginous bone and subchondral bone lesions

A foreign research team conducted a study to observe the levels of lysine oxidase (LOX) and matrix metalloproteinase (MMP) -1, -2, and -3 in the ligament tissues of HA patients and osteoarthritis (OA) patients.
The pathological changes were designed to explore the changes in the levels of lysine oxidase (LOX) and matrix metalloproteinases (MMP)-1, -2, and -3 in the ligament tissues of patients with HA
.

The study used Masson's trichrome staining method, verhoff-van Gieson staining method, and hematoxylin-eosin staining method to observe the basic pathological changes of ligaments
.
Western blot was used to detect the expression levels of type I collagen, elastin, LOXs and MMP-1, -2, -3

The results show that compared with OA ligaments, HA ligaments have looser structures, wider gaps, more breaks, blood cell deposition and local hypertrophy between fibers
.
The expression levels of LOXs and MMPmRNA in HA tissues were up-regulated, which was consistent with the results of western blotting

The study concluded that the ligament metabolism of HA patients is more complicated than that of OA patients, and the ligaments of HA patients have a stronger healing and destruction process
.
This pathology is related to unbalanced inflammatory factors caused by iron overload and repeated intra-articular bleeding

Zheng, L, Luo, D, Li, J, et al.