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    Home > Biochemistry News > Enzyme Technology > Highly active pyrite nanoenzymes and their self-catalyzed anti-tumor research progress

    Highly active pyrite nanoenzymes and their self-catalyzed anti-tumor research progress

    • Last Update: 2021-04-28
    • Source: Internet
    • Author: User
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    bioon.
    com/nanomedicine/">Nanoenzymes are a class of bioon.
    com/nanomedicine/">nanomaterials with their own enzymatic properties.
    Like natural enzymes, they can catalyze enzyme substrates under mild conditions, showing the same enzymatic reaction kinetics and reaction mechanism as natural enzymes, and can be used as Alternatives to natural enzymes are used to detect diseases.
    In recent years, scholars found that
    bioon.
    com/nanomedicine/">nano- enzyme contains an oxidoreductase activity of active oxygen can regulate the cell, the catalytic site of the tumor such as H2O2 to produce hydroxyl radicals, leading to apoptosis of tumor cells.
    However, due to the limited concentration of H2O2 at the tumor site and the low affinity of nanozymes with the substrate H2O2, the generated hydroxyl radicals are usually insufficient to effectively treat tumors.
    The application of nanozymes in tumor therapy currently faces the following two problems: one is how to increase the affinity of nanozymes with the substrate H2O2; the other is how to increase the concentration of H2O2 in
    bioon.
    com/course_video/chang-fei-bian-ma-RNA-yu-zhong-liu959063.
    html">tumor cells.

    bioon.
    com/nanomedicine/">Nano bioon.
    com/nanomedicine/">Nano bioon.
    com/nanomedicine/">Nano bioon.
    com/course_video/chang-fei-bian-ma-RNA-yu-zhong-liu959063.
    html">tumor

    Recently, ACS Nano published online the latest research progress of the team of Yan Xiyun, an academician of the Chinese Academy of Sciences and a researcher at the Institute of Biophysics of the Chinese Academy of Sciences/The Nanoenzyme Engineering Laboratory of the Chinese Academy of Sciences.
    In response to the above problems, researchers designed a new type of nanoenzyme-pyrite (FeS2) nanoenzyme.
    The researchers found that this nanozyme has a very high affinity for binding to H2O2 substrates, and its H2O2 catalyzing efficiency (kcat/KM) is 4144 times higher than that of traditional Fe3O4 nanozymes and 3086 times higher than natural horseradish peroxidase.
    The researchers used first-principles calculation methods to explore the reasons and found that compared with traditional Fe3O4 nanozymes, the covalent bond of pyrite (FeS2) nanozymes when interacting with the substrate H2O2 is shorter, and the surface Contains many gully-like structures, making it more capable of binding to the substrate, so it can catalyze the limited H2O2 at the tumor site, produce a large number of hydroxyl free radicals, and cause
    bioon.
    com/course_video/chang-fei-bian-ma-RNA-yu-zhong-liu959063.
    html">tumor cell apoptosis.

    bioon.
    com/course_video/chang-fei-bian-ma-RNA-yu-zhong-liu959063.
    html">Tumor

    Studies have found that pyrite nanozymes not only have a high affinity for H2O2, but can also produce H2O2 by themselves.
    This is because pyrite nanozymes have the activity of glutathione oxidase, which can oxidize reduced glutathione (GSH) in cells to generate H2O2, and the generated H2O2 can be used as a substrate for peroxidase.
    Therefore, the two enzymatic effects contained in pyrite nanozymes-glutathione oxidase and peroxidase, constitute a self-cascade reaction, which can continuously produce hydroxyl free radicals and cause more
    bioon.
    com/course_video/chang-fei-bian-ma-RNA-yu-zhong-liu959063.
    html">tumor cell apoptosis.
    In addition, the activity of glutathione oxidase can also cause the consumption of GSH, and GSH is a cofactor of the membrane lipid repair enzyme-glutathione peroxidase 4, and its depletion can lead to the inactivation of the latter.
    The lipid peroxides in the cells cannot be effectively eliminated, causing the iron death of the cells.

    bioon.
    com/course_video/chang-fei-bian-ma-RNA-yu-zhong-liu959063.
    html">Tumor

    Pyrite nanozymes have the characteristics of causing tumor bioon.
    com/tags/%E7%BB%86%E8%83%9E%E5%87%8B%E4%BA%A1/">cell apoptosis and iron death at the same time , so that it still shows highly effective tumor treatment effects in drug-resistant tumor cells containing KRAS mutations and apoptosis resistance.
    In addition, the researchers also studied the biological safety of pyrite nanoenzymes, and the results showed that its cell killing effect is tumor-specific.
    This is mainly due to the more vigorous metabolism of tumor cells than normal cells, and more H2O2.
    Nanoenzymes catalyze the production of more hydroxyl free radicals; tumor cells need more GSH to maintain redox balance, so they are more sensitive to GSH depletion; tumor cells need more iron ions for rapid growth, so iron death is more likely to occur.
    Pyrite nanoenzyme has good biodegradability,
    bioon.
    com/course_video/chang-fei-bian-ma-RNA-yu-zhong-liu959063.
    html">tumor- specific killing effect and biodegradability provide safety guarantee for its in vivo application.

    bioon.
    com/tags/%E7%BB%86%E8%83%9E%E5%87%8B%E4%BA%A1/">Apoptotic bioon.
    com/course_video/chang-fei-bian-ma-RNA-yu-zhong-liu959063.
    html">tumor

    The highly active pyrite nanoenzyme overcomes the problem of low affinity of traditional peroxidase nanoenzymes for H2O2, and can catalyze the production of a large number of hydroxyl radicals from the limited H2O2 at the tumor site.
    The discovery of glutathione oxidase activity, a new activity of pyrite nanozymes, can not only provide substrate H2O2 for its peroxidase activity, but also catalyze the oxidation of glutathione and cause cell iron death.
    So as to realize tumor apoptosis-iron death synergistic treatment.
    What is important is that pyrite nanozymes do not need other help, and a single material can achieve efficient and safe
    bioon.
    com/course_video/chang-fei-bian-ma-RNA-yu-zhong-liu959063.
    html">tumor treatment effects, which shows that it has clinical transformation potential.
    (
    bioon.
    com">Bioon.
    com)

    bioon.
    com/course_video/chang-fei-bian-ma-RNA-yu-zhong-liu959063.
    html">Tumorbioon.
    com">

     

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