How to distinguish the copy number variation of heterogeneous samples [Innovative Tips]

Many biological samples are heterogeneous, such as stem cell cultures and tumor samples, which are composed of different cell types

Experts at Bio-Rad believe that if you want to obtain reliable data from mixed cultures, the most effective method is to sort the target cells first, and then perform the measurement

First, they use the S3 or S3e cell sorter to sort heterogeneous populations based on cell morphology or fluorescent markers

This time, they studied copy number variation (CNV), which is the amplification or deletion of genes in the genome, which plays an important role in cancer, neurological diseases, and drug metabolism

In this study, the researchers used HEK 293-GFP and ATCC HTB-70 cell lines

After sorting, the researchers found that the purity of the enriched HEK 293-GFP cells reached 99.

The author believes that the combination of flow cytometry, cell sorting, and digital PCR can bring highly accurate and specific results

Learn more about the applications of digital PCR technology

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Discriminating Copy Number Variation from Heterogeneous Samples Using the S3™ Cell Sorter and the QX200™ Droplet Digital™ PCR System Application Note