Instead of PCR, RPA plays an important role in HIV testing

Biocom reports: Recombinase polymerase amplification of RPA is known as a nucleic acid detection technology that can replace PCR .

In HIV diagnosis and treatment, immediate testing is very important, especially for babies

The current gold standard method for infant HIV diagnosis is DNA PCR , but many regions do not have such conditions

Paper HIV DNA detection device

2012 In September researchers developed a device made of paper, plastic and glass fiber, used to HIV 's DNA for RPA amplification

People can first extract DNA from dried blood spot samples of babies , and then perform RPA amplification

The actual effect of RPA in detecting HIV DNA

If the HIV-1 in the infant cannot be detected in time , the treatment will be delayed and the treatment effect will be affected

This is an isothermal amplification method that can detect all major subtypes of HIV-1 , and can help people diagnose HIV- infected babies

Real-time quantitative detection of HIV DNA

In May of this year, researchers used internal controls and standard curves to quantify the concentration of HIV-1 DNA through RPA amplification .

This study analyzed and quantified HIV-1 DNA samples

Unplugged HIV DNA test

Most nucleic acid amplification (including isothermal amplification) requires electricity

A study in September of this year showed that when the room temperature is between 31°C-43°C , the RPA reaction can be successfully incubated for 20 minutes , and 10 copies of HIV-1 proviral DNA can be detected .

When the room temperature drops below 30°C , the amplification performance of RPA decreases

Researchers at 15 [deg.

Biological Communication Editor: Ye Yu