Instead of PCR, the application of RPA in cancer research

Biocom reports: Recombinase polymerase amplification of RPA is known as a nucleic acid detection technology that can replace PCR .

More than 40 years have passed since mankind "declared war" on cancer, and this malignant disease is still a lingering shadow

RPA for cancer mutation detection

The Singapore A*STAR research team published in Lab on a Chip magazine last June , a new technology that can quickly detect single point mutations in cancer, Isothermal Solid Phase Amplification / Detection ( ISAD )

In this study, ISAD was mainly used to detect single point mutations in the HRAS ( Harvey RAS ) gene

Studies have shown that the sensitivity of ISAD technology is one hundred times higher than that of RPA and traditional PCR methods .

RPA is used for anti-cancer drug screening

Last October, a cancer study using RPA technology was also published in Talanta magazine .

An aptamer is a single-stranded short DNA .

The researchers optimized on the basis of BBC analysis, using Cyto-c specific aptamers, and replacing the time-consuming PCR with isothermal recombinase polymerase amplification ( RPA ) .

Biological Communication Editor: Ye Yu