JE: Zuojin Wan relieves Helicobacter pylori-induced chronic atrophic gastritis

Background: chronic atrophic gastritis (CAG) is a common digestive diseases, Helicobacter pylori infection can induce the CAG, can eventually lead to gastric cancer
.

Zuojin Wan comes from the "Danxi Heart Method" and is composed of Coptis and Evodia according to 6:1 .

Chronic atrophic gastritis (CAG) chronic atrophic gastritis (CAG) digestion infected gastric Zuojinwan Zuojinwan berberine, Evodia by 6: 1 berberine, Evodia by 6: 1 digest commonly used prescriptions commonly used prescription

Research method: Establish a CAG model of SD rats induced by Helicobacter pylori (control group, model group, Zuojin pill low, medium, and high dose groups, omeprazole group), and detect rat’s gastric pathology and serum inflammatory factors The level of inflammation and mucosal damage
.

The GES-1 cell model was used to detect the improvement of Zuojin's cell viability and morphology

Helicobacter pylori induced SD rat CAG model Helicobacter pylori induced SD rat CAG model Gastric histopathology Gastric histopathology Serum inflammatory factors Serum inflammatory factors JMJD2B/COX-2/VEGF axis and HMGB1/NF-κB signaling pathway JMJD2B/ COX-2/VEGF axis and HMGB1/NF-κB signaling pathway

Research results: The serum levels of IL-6, MCP-1, PGE2, TNF-α, and VEGF in the model group were significantly higher than those in the control group
.

Compared with the model group, the Zuojinwan high-dose group (2.

Pathological observations showed that the mucosa of the control group was intact, with tight, abundant, and orderly gastric glands
.

In CAG model rats, the proper glands are missing, the mucosa partly falls off, and the mucosa neutrophils infiltrate

Edema, congestion, erosion, and atrophy are significantly improved Edema, congestion, erosion, and atrophy are significantly improved

The results of CCK-8 method to detect GES-1 cell viability showed that 60 μg/mL Zuojin Pills had a better protective effect on cells, and the optimal concentration was used to investigate its protective effect
.

In addition, Helicobacter pylori infection with lower MOIs (10:1, 20:1, 50:1) resulted in a dose-dependent expression of JMJD2B.

Promote the proliferation of GES-1 cells, improve the gastric epithelial cell damage induced by Helicobacter pylori

The results of rat immunohistochemistry showed that the expression levels of JMJD2B and COX-2 in the model group were higher than those in the control group.
Zuojinwan treatment reduced the expression levels of JMJD2B and COX-2
.

The results of in vivo and in vitro RT-qPCR and western blotting showed that Helicobacter pylori infection can significantly increase the relative expression of JMJD2B, COX-2, VEGF, VEGFR1 and VEGFR2 protein and mRNA compared with the control group

immunity

In addition, Helicobacter pylori can significantly increase the relative expression of HMGB1, MyD88 and RAGE mRNA in rats and GES-1 cells
.

Zuojinwan treatment reduced the expression levels of these genes, among which the high-dose group was the most significant

Research conclusion: Zuojin Pill inhibits Helicobacter pylori-induced inflammatory response through JMJD2B/COX-2/VEGF axis and HMGB1/NF-κB signaling pathway , thereby preventing gastric mucosal damage
.

The results of this study suggest that the potential role of Zuojin Pills in the treatment of CAG needs to be further studied

Inhibit Helicobacter pylori-induced inflammatory response, thereby preventing gastric mucosal damage.
Inhibiting Helicobacter pylori-induced inflammatory response, thereby preventing gastric mucosal damage

Literature source:

Wen J, Wu S, Ma X, Zhao Y.
Zuojin Pill attenuates Helicobacter pylori-induced chronic atrophic gastritis in rats and improves gastric epithelial cells function in GES-1 cells [published online ahead of print, 2021 Nov 19].
J Ethnopharmacol.
2021;114855.
doi:10.
1016/j.
jep.
2021.
114855

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