Lipid staining method.

fats and lipids (phospholipids, glycolipids, steroids, etc.) are collectively referred to as lipids. It is the normal
of
tissues, insoluble in water and soluble in alcohol, ether, chloroform and other lipid solvents. In chemical composition, lipids belong to fatty acids or substances associated with these esters. The main function of lipids is oxidation to supply energy.
fat is mainly found in adipose tissue and is present in fat cell pulp with oil droplets.
in pathological tests, lipid staining is most commonly used to prove fat degeneration, fat hydrants, and tumor identification. The most widely used dyes for lipid dyes are Sudan dyes, the most commonly used are Sudan III., Sudan IV., Sudan Black and Oil Red O, etc. Fat is dyed, and in fact Sudan dyes are absorbed by fat dissolving and rendering the color of the dye. It is concluded that lipids in tissues are best colored for Sudanese dyes when they are liquid or semi-liquid. According to this principle, an appropriate increase in temperature (37 degrees C-60 degrees C) is beneficial
the
dyeing effect of tissue slices.
lipids, sliced with frozen or paraffin, sealed with water-soluble sealing agents, such as glycelin gelatin and Arabic sugar gum.
, Sudan III. Staining Law: . How to do it:
(1) is fixed to 10% formaldehyde tissue.
(2) washed and sliced with ice or paraffin.
(3) after distilled water, soaked in Harris sulmin or alum suminin lightly dyed for 1-2 minutes.
(4) tap water.
(5) after washing, remove 70% alcohol for 5 seconds.
(6) is placed in a temperature tank at 56 degrees C for approximately 30 minutes or more in Sudan III.
(7) wash in 70% alcohol for 5-10 seconds.
(8) is washed in distilled water and the slices are moved to the slide.
(9) slice on the slide and carefully wipe off the moisture around the slice.
(10) glycesterol sealed.
results: fat is orange red or bright red or black, bile lipids are pale red, fatty acids are not colored, and the nucle nucles are blue.
reagents
preparation:
1, Sudan III. Dyeing method: dissolve 0.15 grams of Sudan III. Dissolve in 100 ml of 70% alcohol or pure acetone and 70% alcohol mixture (50ml each),
filtration
, the resulting filter is saturated concentration.
: When dyed, the container must be covered, otherwise alcohol or acetone volatile, dye precipitation.
2, glycelic gelatin preparation:
gelatin 40g
distilled water 210ml
glycelic 250ml
stone carbonic acid crystallization 5ml
first immerse gelatin in distilled water for 2 hours or more, then add glyceline and statin,
heating
15 minutes, shake until mixing.
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