Lipid staining.

I. Fat overview
lipids in the human body occupies a considerable part, usually they exist in two forms in the human body, the first is fat, mainly neutral fat, storage sites such as the epithelial, large mesh, intercellular kidney
tissue
and other parts, the other is structural fats, such as phospholipids, cholesterol and other proteins, sugars together in the cell composition.
1, pure lipids: by the fatty acids of alcoholized esters, such as neutral fats, oils and waxes, Sudan dyes can be dyed black or red.
2, compound substances, fatty acid esters hydrolyze to produce alcohols, fatty acids, phosphoric acids and nitrogen-containing bases, compound lipids are divided into phospholipids and glycolipids.
(1) phospholipids: lecithin, cerebral phospholipids, and neurodegree phospholipids. Sudan black stain is positive and PAS reaction is negative.
(2) saccharin, PAS tested positive.
(3) derived lipids: refers to pure lipids or compound lipids that are water products that are also lipid properties and belong to such fatty acids and sterols.
fatty acids: sulfate Nel blue positive, PAS negative.
cholesterol and its esters: Schultz tested positive.
lipids:
1, neutral substances such as triglycerides, cholesterol and their esters, steroids and certain glycerides.
2, acidic lipids, fatty acids, phospholipids.
II, Sudan III IV joint law (combined sudan III IV for lipids
1, lipid tissue fixed and fixed liquid
lipid tissue fixed special attention to the problem is not to choose alcohol-containing fixed liquid, because alcohol can dissolve lipids, therefore, the preferred fixation for lipids is formaldehyde, it can better preserve lipids.
2,
reagents
preparation: Sudan III0.2g, 70% alcohol 50ml; Sudan IV0.5g acetone 50ml.
3, operating steps:
(1) with formaldehyde-fixed tissue for freezing
slice
, about 10-15um thick, cut and put into distilled water, dyeing before 50%-70% alcohol washing.
(2) into Sudan III IV dyeing 5 minutes,
(3) 70% alcohol differentiation,
(4) washing,
(5) sumicin shallow-dyed cell nucleus,
(6) washing, if necessary differentiation,
(7) blue, if floating dyeing, at this time should be attached to the film,
(8) glycerin seal.
result: lipids are orange or bright red, nuclear blue.
.