Methods for the production of feed enzymes

feed enzymes are generally divided into a single enzyme preparation and compound enzyme preparation twoE5U
1Production method of single enzyme preparationE5Usingle enzyme preparation is a combination of pure enzyme preparation plus carrier or thinner, the industrial production of pure enzyme preparation is generally produced by microbial liquid fermentation method, the method is to use liquid medium in the volume of 50 to 100m3 fermentation tank, ventilation and culture, to the full growth of microorganisms and synthesis of a large number of microbial enzymes, the enzyme is isolated, purification, and make a certain form of enzymeThe separation and extraction of enzymes needs to go through the whole process of cell crushing, extraction, centrifugation, filtration, concentration, drying, etcThe advantages of this method: (1) the liquid medium is highly mobile, the process condition control is easier, is conducive to automatic production, (2) because of the fermentation of pure bacteria in the closed fermentation tank, so the quality of enzyme purity is also stable, (3) has a high degree of mechanization, low labor intensity, high equipment utilization advantagesIts disadvantages: (1) less enzyme production; (2) incomplete components of enzymes; (3) energy consumption, (4) more difficult separation extraction, (5) more and more complex equipment, investment, not easy to get on the horse and so onE5U
2Feeding compound enzyme production methodsE5Uis well known, because of the impact of livestock and poultry with feed digestion factors are many, the role of single enzyme as an additive is bound to be very limited, in order to better improve the nutritional value of feed and livestock digestion capacity, make full use of the synergy of enzymes, play its comprehensive effect, while using a variety of enzymes compound use is very important, so in the past decade, the world generally developed a multi-enzyme system for feeding, the production of compound enzyme productsE5U
　　There are two ways to produce compound enzymes that meet the requirements of the formula: E5U 1) full yupting method E5U production or purchase of a variety of single enzyme preparations, according to the formulation of compounding, plus dispersant, thinner This method only requires reliable enzyme quality and very precise formulation execution It has many disadvantages, commodity pure enzyme preparation costs are already very high, such as using a variety of pure enzyme preparations for compound ingenuity, the cost of preparation of feeding compound enzymes will be higher, limiting its application, and then this method of production of feeding compound enzymes is difficult to obtain in addition to enzyme function can promote animal growth of some unknown growth factors E5U
　　2) Solid fermentation method E5U solid fermentation method also known as bran culture method, the method is the use of bran or rice bran as the main raw material, in addition to the need to add other ingredients, add water mixed into a moderate amount of water solid material as a medium for microbial reproduction and enzyme production Solid fermentation method is also an ancient process, its disadvantages: easy to mixed bacteria pollution, so the enzyme purity is poor, the utilization rate of solid raw materials is low, but also because the condition control of solid fermentation is not uniform, the quality of the produced enzyme is difficult to stabilize, the production of labor intensity, occupy more sites It also has many advantages: (1) high enzyme production in unit volume culture equipment; (2) power saving; (3) less problems caused by pollution; (4) enzyme extraction concentration can be arbitrarily adjusted; (5) less after-treatment equipment; (6) less investment, easy to master technology, easy to get on the horse The study of solid fermentation is attracting renewed attention With the development and progress of biotechnology and fermentation industry, the shortcomings of solid fermentation will be gradually overcome Domestic traditional brewing products such as liquor, soy sauce production of wine, soy sauce, wine industry glycifying, etc are using solid fermentation This method is suitable for the production of enzyme preparations using mold Solid fermentation method has the following two specific methods: E5U (1) single-bacterial solid fermentation, supplementing enzyme method using a single strain fermentation, and then with a certain type, a certain amount of pure enzyme preparation, its enzyme composition has improved, but the cost increased E5U
　　(2) Multi-strain mixed solid fermentation, natural complementary enzyme system most microbial strains only one enzyme active, so that several strains of mixed fermentation, complementary enzyme production, so that each strain of maximum enzyme production performance to play out, the production of enzymes not only composed of comprehensive, high activity, strong function, but also low cost, the application effect is very good, but the technology is very difficult E5U feed enzymes are generally divided into single enzyme and compound enzyme E5U
　　1 Production method of single enzyme preparation E5U single enzyme preparation is a combination of pure enzyme preparation plus carrier or thinner, the industrial production of pure enzyme preparation is generally produced by microbial liquid fermentation method, the method is to use liquid medium in the volume of 50 to 100m3 fermentation tank, ventilation and culture, to the full growth of microorganisms and synthesis of a large number of microbial enzymes, the enzyme is isolated, purification, and make a certain form of enzyme The separation and extraction of enzymes needs to go through the whole process of cell crushing, extraction, centrifugation, filtration, concentration, drying, etc The advantages of this method: (1) the liquid medium is highly mobile, the process condition control is easier, is conducive to automatic production, (2) because of the fermentation of pure bacteria in the closed fermentation tank, so the quality of enzyme purity is also stable, (3) has a high degree of mechanization, low labor intensity, high equipment utilization advantages Its disadvantages: (1) less enzyme production; (2) incomplete components of enzymes; (3) energy consumption, (4) more difficult separation extraction, (5) more and more complex equipment, investment, not easy to get on the horse and so on E5U
　　2 Feeding compound enzyme production methods E5U is well known, because of the impact of livestock and poultry with feed digestion factors are many, the role of single enzyme as an additive is bound to be very limited, in order to better improve the nutritional value of feed and livestock digestion capacity, make full use of the synergy of enzymes, play its comprehensive effect, while using a variety of enzymes compound use is very important, so in the past decade, the world generally developed a multi-enzyme system for feeding, the production of compound enzyme products E5U
　　There are two ways to produce compound enzymes that meet the requirements of the formula: E5U 1) full yupting method E5U production or purchase of a variety of single enzyme preparations, according to the formulation of compounding, plus dispersant, thinner This method only requires reliable enzyme quality and very precise formulation execution It has many disadvantages, commodity pure enzyme preparation costs are already very high, such as using a variety of pure enzyme preparations for compound ingenuity, the cost of preparation of feeding compound enzymes will be higher, limiting its application, and then this method of production of feeding compound enzymes is difficult to obtain in addition to enzyme function can promote animal growth of some unknown growth factors E5U
　　2) Solid fermentation method E5U solid fermentation method also known as bran culture method, the method is the use of bran or rice bran as the main raw material, in addition to the need to add other ingredients, add water mixed into a moderate amount of water solid material as a medium for microbial reproduction and enzyme production Solid fermentation method is also an ancient process, its disadvantages: easy to mixed bacteria pollution, so the enzyme purity is poor, the utilization rate of solid raw materials is low, but also because the condition control of solid fermentation is not uniform, the quality of the produced enzyme is difficult to stabilize, the production of labor intensity, occupy more sites It also has many advantages: (1) high enzyme production in unit volume culture equipment; (2) power saving; (3) less problems caused by pollution; (4) enzyme extraction concentration can be arbitrarily adjusted; (5) less after-treatment equipment; (6) less investment, easy to master technology, easy to get on the horse The study of solid fermentation is attracting renewed attention With the development and progress of biotechnology and fermentation industry, the shortcomings of solid fermentation will be gradually overcome Domestic traditional brewing products such as liquor, soy sauce production of wine, soy sauce, wine industry glycifying, etc are using solid fermentation This method is suitable for the production of enzyme preparations using mold Solid fermentation method has the following two specific methods: E5U (1) single-bacterial solid fermentation, supplementing enzyme method using a single strain fermentation, and then with a certain type, a certain amount of pure enzyme preparation, its enzyme composition has improved, but the cost increased E5U
　　(2) Multi-strain mixed solid fermentation, natural complementary enzyme system most microbial strains only one enzyme active, so that several strains of mixed fermentation, complementary enzyme production, so that each strain of maximum enzyme production performance to play out, the production of enzymes not only composed of comprehensive, high activity, strong function, but also low cost, the application effect is very good, but the technology is very difficult E5U
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