Nat Aging: What are the healthy aging characteristics of human monocytes?

Even in healthy people, old age is accompanied by a gradual decline in cognitive, metabolic and physiological abilities and can increase neurodegenerative, cardiovascular and chronic inflammatory disease susceptivity.
time, it is often difficult to determine whether age-related markers reflect changes in individual cells or changes in cell type abundance, especially when full tissue transcription or exogentation is performed.
Despite the large amount of clinical and epidemiological data, little is known about the nature of age-related changes in specific primary cell groups in healthy individuals, particularly in the age-related changes in the surface genetic landscape.
To directly address this problem, the researchers focused on the classic CD14-CD16-monocytes because they are homogeneous, easy to obtain, and relatively abundant in the blood, allowing researchers to obtain multiple histological profiles of these cells from a single blood draw.
of aging can be manifested in two key areas: age-related chromatin modification changes and DNA methylation changes.
the link between aging and DNA methylation has been well recognized, however, despite extensive research, age-related DNA methylation/de-methylation cell-specific regions have not been reported to date.
here, the researchers report on the full features of healthy aging of classical human monocytes, focusing on changes in the exogenomic genome, transcriptions, and proteomics, as well as corresponding proteomic and plasma metabolic data.
the study used healthy queues of 20 young and 20 older men (average ages about 27 and about 64).
For each individual, the researchers performed enhanced reduction-characterized bisulphate sequencing based on DNA methylation spectrum, which enabled the researchers to identify a new, cell-specific aging characteristic in a group of age-related differential methylation regions (DMRs) -- DNA methyl groups.
low methylation events were associated with H3K27me3 in CpG Island near the low expression gene initiater, while low methylation DMRs were found in areas labeled H3K4me1 and were associated with an increase in age-related expression of the corresponding gene.
, the study provides a link between DNA methylation in primary cells and age-related transcriptional changes.
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