OMCL: Yishen Paidu Decoction protects diabetic kidney damage by inhibiting oxidative stress, inflammation and epithelial-mesenchymal transition in mice

Purpose: Diabetic nephropathy (DKD) is one of the most common chronic microvascular complications of diabetes, but there is still a lack of effective treatment strategies
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Yishen Paidu Recipe (YSPDF) is a traditional Chinese medicine preparation that has been clinically used to treat chronic kidney disease (CKD) for more than 20 years

Yishen Paidu Recipe (YSPDF) is a traditional Chinese medicine preparation that has been clinically used to treat chronic kidney disease (CKD) for more than 20 years

Results: Yangshen Paidu Decoction can significantly improve the various biochemical indicators of rats (fasting blood glucose, blood creatinine, blood urea nitrogen, 24h urine total protein, total cholesterol, total triglycerides), improve renal pathological changes and renal tissue fiber
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In addition, it can significantly inhibit the oxidative stress state and the levels of inflammatory cytokines (TNF-α, IL-6, IL-1β and monocyte chemoattractant protein-1)

Various biochemical indicators (fasting blood glucose, blood creatinine, blood urea nitrogen, 24h urine total protein, total cholesterol, total triglycerides), improve renal pathological changes and renal tissue fibrosis, significantly inhibit oxidative stress and inflammatory cytokines (TNF-α, IL-6, IL-1β and monocyte chemoattractant protein-1) levels significantly reduce renal fibrosis, and inhibit transforming growth factor-β-1/Smad pathway to inhibit epithelial to mesenchymal transition.

Table YSPDF can improve the level of various biochemical indicators of db/db mice
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Table YSPDF can improve the level of various biochemical indicators of db/db mice
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Figure 1 The effect of Yangshen Paidu Recipe on histopathological changes of mouse kidney
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(A) Typical images (400 times) of HE staining, PAS staining and Masson staining of kidney tissue

Figure 1 The effect of Yangshen Paidu Recipe on histopathological changes of mouse kidney

Figure 2 YSPDF can improve the oxidative stress state of db/db mice
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(A) Oxidative stress parameters include reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT)

Figure 2 YSPDF can improve the oxidative stress state of db/db mice

Figure 3 YSPDF can inhibit the production of inflammatory factors in INB/DBM mice
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ELISA was used to detect the pro-inflammatory factors TNF-α, IL-6, IL-1β, monocyte chemoattractant protein-1 (MCP-1), compared with the normal control group #p<0:05 and ##p<0 ：001, compared with diabetic nephropathy group∗p<0:05, ∗∗p<0:01, ∗∗∗p<0:001

Figure 3 YSPDF can inhibit the production of inflammatory factors in INB/DBM mice

Our research shows that Yangshen Paidu Decoction may reduce kidney damage in db/db mice by inhibiting oxidative stress, inflammation and EMT, and its mechanism may be related to the activation of Nrf2 pathway
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Original source:

Zhang Q, Liu X, Sullivan MA, Shi C, Deng B, db/dbProtective Effect of Yi Shen Pai Du Formula against Diabetic Kidney Injury via Inhibition of Oxidative Stress, Inflammation, and Epithelial-to-Mesenchymal Transition in Mice.
Oxid Med Cell Longev 2021; 2021 

db/dbProtective Effect of Yi Shen Pai Du Formula against Diabetic Kidney Injury via Inhibition of Oxidative Stress, Inflammation, and Epithelial-to-Mesenchymal Transition in Mice.
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