Microbial fermentation system operating procedures

pre-fermentation preparationcheck that the power supply is correct and that the compressor, microcomputer system and circulating water system are working properlyCheck that the valves, connectors and fastening screws on the system are tightenedOpen air compressor, with 0.15Mpa pressure, check the seed tank, fermentation tank, filter, pipeline, valve and other sealing is good, there is no leakageWhether the tank jacket is sealed (with emphasis on inspection during the season) ensures that all valves are closed (except for valves in front of the solenoid valve)Check whether water (cooling water) pressure, voltage, gas (vapor) pressure can be supplied normallyInlet pressure is maintained at 0.12Mpa, allowing change slower than 0.3Mpa in the 0.15-0.2Mpa range, temperature should be lower than the fermentation temperature of 10 degrees C; Body reliable grounding, input steam pressure should be maintained at 0.4Mpa, decompression after entering the system is 0.24MPa, air compressor pressure value 0.8Mpa, air inlet pressure should be controlled at 0.25-0.30MP (air primary filter pressure value)"text-align: center;"img src""alt"""microbial fermentation system operating procedures" title""microbial fermentation system operating procedures" -temperature, dissolved oxygen electrode, PH electrode correction and calibration, see touch screen PH, DO calibration helpcheck whether each motor is functioning properly (4 out of 1)Whether the solenoid valve can be sucked in properly (a total of 11 solenoid valves in the complete system)sterilizedfermentation system installed after the first cleaningtank cleaning: acid-base tank, filling tank, seed tank can be the cylinder above the flange removed, by the operator using a clean cloth manual cleaning, after the end of the discharge of sewage in the tank, in more than a few times can be rinsedThe cleaning of the fermentation tank can be flushed through a water pipe through a hand hole to the wall of the tank, and when the water level rises to the second impeller of the mixing shaft, stop the flushing and start the motor stirring and cleaningThe cleaning of each line, you can first use clean water flushing, and then according to the corresponding function of the corresponding cleaning media (cleaning the pipeline should be to protect the various components in the pipeline as a prerequisite), the specific steps can refer to "air pipe sterilization." If the fermentation system for a long time or cultured bacteria and the previous batch is not the same, can be used 2% NaOH cleaning, other tanks can also be used in the fermentation tank cleaning method, after cleaning should be sterilized fermentation systemthe sterilization of air lines the decontamination filter on the air line, using steam through the pressure relief valve (air relief valve can not be steamsterized, so the air pre-filter is not sterilized), steam filter and then into the sterilization filter The filter element of the air excitatic filter cannot withstand high temperature and pressure, so the steam relief valve is adjusted to 0.13MPa and not more than 0.15MPa During the empty ingenoidation process, the exhaust valve at the lower end of the sterilizing filter should be slightly opened to exclude condensate The empty time should last about 30 minutes, when the equipment is first used or long-term use after starting, it is best to use intermittent empty, that is, after the first empty, every 3 to 5 hours after the empty once again, in order to eliminate spores After the air-cancelled filter, should be air blow-dry, about 20 to 30 minutes, and then the air way valve closed Keep the air ducts positive the fermentation tank empty (seed tank, feed tank, acid-alkali tank empty as a reference basis) before the fermentation tank empty, the open sewage valve Q22 should be opened (so that the pressure inside the jacket does not exceed pressure) Close Q14, slightly open Q15, open steam valve Q12, open Q13 to the tank to flow steam, open Q18, Q19 through sampling mouth to the tank to pass steam The tank on the inoculation port, exhaust valve Q9, and the sewage line valve Q24, Q25 slightly open, so that steam through these valves, when the humidity reaches 1220C began to time, adjust the opening of the valve Q9, Q13, Q19, to maintain the temperature of the tank 1220C to 1280C (pressure for general lying at 0.11 to 0.15Mpa process pressure adjustment and air pressure) When the time reaches 30 to 40 minutes, close Q13, Q15, Q19, and then close Q12, Q18, open the air line valve Q14, Q13 to the tank air cooling, so that the tank to maintain positive pressure between 0.03MPa-0.05MPa When rapid cooling is required, close the jacket sewage valve Q22, open the cooling water valve Q27/DC1 to the jacket to cool through the water, and close the cooling water when it reaches room temperature In special cases, intermittent emptying can be used (3 to 5 hours apart and then empty again, in order to eliminate spores) When is empty, the dissolved oxygen and PH electrodes are removed and properly preserved to extend their service life fermentation tank reduction (seed tank, filling tank, acid-base tank as reference basis) is the process of sterilizing the medium with steam when the medium is added to the tank After the end of the empty, close the intake valve Q13, open Q9 discharge the pressure in the tank, will be corrected PH, DO electrode installed, as soon as possible the matching medium from the feeding port added to the tank the medium before entering the tank, should first paste, the general medium formula volume should be determined according to process requirements, the final volume of fermentation liquid for the tank's total volume of about 70% calculation (foam more medium for about 60%, foam less medium up to 75 to 80%), taking into account condensate and inoculation factors, as well as whether to increase, the initial capacity by the production process according to the requirements of the production process, need to be explored in practice turn on the mechanical mixing device and turn at low speed so that the material in the tank is mixed evenly open jacket sewage valve Q22, steam valve Q21, pre-warm the inner medium (preheating with the chuck.) When the temperature in the tank rises to 90 degrees C (the specific temperature should vary according to the steam quality), close the jacket inlet valve Q21, close Q14, fully open the inlet valve Q12, open Q13 into the steam, fully open Q18, micro Open Q19 through the sampling port to the tank through steam, off Q25, Q26, fully open Q23, micro-open Q24 to the tank through steam, micro-open exhaust valve Q9 (three-way steam sterilization), shut down the motor stirring When the temperature rises to 121-123 degrees C and the tank pressure rises to 0.12MPa, the opening of the steam valve Q9, Q19, Q13, Q24 is controlled, the temperature and tank pressure are maintained, and the time is started, and the micro-opening flame drains the steam outwards When the time reaches 30 minutes, close the flame inoculation port, close Q24 (before closing should artificially open a large amount of steam several times, to avoid the media remain at the valve), close Q23, close Q19, 18, close Q13, Q12 stop the supply of gas close the jacket sewage valve Q22, open the cooling water valve Q27/DC1 to the jacket water cooling, open the motor stirring, when the pressure drops to 0.05MPa open the air valve Q14, Q13 to the tank through the air, speed up the cooling speed, and keep the tank pressure to 0.05MPa, until the tank culture temperature drops to the inoculation temperature Close valve Q27/DC1 to stop the cooling water when the temperature in the tank drops to 2-3 degrees C higher than the temperature required by the fermentation process seed cans, transfer inoculation: the system is immunized with flame sealing, should be prepared in advance before vaccination: alcohol cotton, pliers, tweezers, vaccination rings, asbestos mesh gloves, Z-word wrench The bacteria are loaded into the triangular flask and the amount of vaccination is determined according to the process requirements The alcohol cotton surrounded by the vaccination ring lit, the bacteria bottle mouth on the flame burned for a while, with a Z-word wrench unscrewed the inoculation port, quickly pour the bacteria into the tank, at this time should be aired to the tank, so that the inoculation mouth has air discharge (pressure close to zero, but not zero) Tighten the inoculation cap after sterilizing on the flame After vaccination, the tank pressure is kept at 0.03MPa to 0.07MPa, adjusting the ventilation volume transfer: should drain the condensate in the steam line before the transfer, after draining to keep the steam valve slightly open (only flowing water does not drain steam) so that the condensate in the steam line can be drained at any time When moving the plant operation, special attention should be paid to adjust the pressure difference between the seed tank and the culture tank, the pressure of the seed tank should be higher than the culture tank 0.1MPa in order to transfer! When specgearing is closed, the steam valve should be removed immediately to avoid negative pressure contamination Immediately after the removal of species, and then open the steam valve connected with the steam into the steam for flushing disinfection, and immediately clean the seed tank, to prevent fermentation material bonding to the pipe valve and tank wall, disinfection immediately after the closure of the steam valve and discharge condensate valve and tube cap sampling can be sampled at the sampling port after vaccination is completed or during fermentation Before sampling, the sampling line valve needs to be sterilized with steam to prevent contamination of the bacteria and cause misleading, after sampling also to use steam to flush the sampling pipe and valve operation method: full-open steam valve Q18, micro-open Q20, when the time to 15 minutes, the alcohol cotton surrounded by the sampling port lit, close Q20, close Q18, open Q19, open Q20, After draining the liquid from the sampling tube, open the prepared sampling bottle at the edge of the flame, quickly sample and cover, close the Q20, then open the Q18 cleaning/sterilization sampling port for 5 minutes, close Q20, Q18 After sampling is completed, the PH value of the fermentation solution should be detected with ph meter, and then the PH is corrected, when the system is completed, adjust the pressure and flow, the system can enter fermentation mode, automatic control (temperature, PH, DO), the specific process parameters are set by the operator himself, the system will be set according to the set parameters can be automatic control or manual control of the operator fermentation culture sampling is completed, the PH value of fermentation solution should be detected with ph meter, and then corrected PH; Seeds into the seed tank or fermentation tank after temperature control culture, tank pressure generally 0.03-0.05Mpa, speed according to the process requirements, adjust the temperature of circulating water to control the fermentation temperature, when the ambient temperature is higher than the fermentation temperature, with condensate cooling; PH, DO REGULATION BY THE CONTROL SYSTEM THROUGH THE IMPLEMENTATION OF THE MECHANISM TO AUTOMATICALLY ADD AND SUBTRACT THE REALIZATION The foam alarm is detected by the foam probe as a foam signal on the touch screen as an indicator note: 1) before sterilization should be PH electrode correction, the control of PH in the culture process using the peristaltic pump acid plus alkali to achieve, acid bottle base bottle first in the steriliser 2) the measurement and control of dissolved oxygen (DO) the calibration of dissolved oxygen electrodes: before vaccination at a constant fermentation temperature, the speed and air volume to the maximum when the dissolved oxygen DO value as 100% the measurement and control of dissolved oxygen in the culture process: the control of DO value is achieved by regulating the air flow and the speed of the dissolved oxygen Second, the intake (manual) control must be adjusted simultaneously out the material of this equipment is the use of tank pressure to discharge the fermentation liquid from the discharge pipe, according to the concentration of the fermentation fluid, tank pressure can be controlled at 0.05 to 0.1MPa after the extraction of dissolved oxygen, PH meter, cleaning and maintenance after the discharge, immediately put water to clean the fermentation tank and the pipe valve, and open the air compressor, supply gas to the fermentation tank and stir, the fermentation liquid in the pipeline rinse if the fermentation tank is temporarily unused, the fermentation tank is emptied and the water in the tank, jacket and pipe is emptied