Protein Cell: Joint report by Chinese scientists: New coronavirus RNA does not have the ability to integrate into the host genome

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is an RNA virus in the Coronavirus family, which caused the outbreak of new coronary pneumonia in 2019 and a global pandemic
.

Although a variety of vaccines are currently available, which brings hope to human society to alleviate and ultimately prevent the epidemic, more in-depth research is still needed to understand the pathogenesis of the virus, especially the interaction between the virus and the host, in order to develop effective Intervention measures

According to the type of genome to which the virus belongs, viruses are mainly divided into four categories, including dsDNA, ssDNA, dsRNA and ssRNA
.

All viruses need host cells to reproduce.

Recently, the research team of the Chinese Academy of Sciences and the Chinese Academy of Medical Sciences jointly published an online article entitled "Comprehensive analysis of RNA-seq and whole genome sequencing data reveals no evidence for SARS-CoV-2 integrating into host genome" in Protein&Cell, revealing SARS -CoV-2 RNA does not have the ability to integrate into the host genome
.

http: //doi.
org/10.
1007/s13238-021-00861-8

First, the research team performed ribonucleic acid sequence analysis on 293T, Huh-7 and Calu-3 cells infected with the new coronavirus, and identified 347, 3107, and 4171 chimeras from 293T, Huh-7 and Calu-3 cells, respectively.
Gene
.

Most of it is the fusion of viral RNA and host cell mRNA

As a result, the researchers further obtained 132 conserved chimeric genes from all three cell lines and analyzed the types of RNA
.

The results showed that the chimeric gene is preferentially mRNA, which may be due to the rich expression of mRNA relative to other RNA types

Common chimeric genes in human ribonucleic acid and new coronavirus ribonucleic acid

Common chimeric genes in human ribonucleic acid and new coronavirus ribonucleic acid Common chimeric genes in human ribonucleic acid and new coronavirus ribonucleic acid

Subsequently, the researchers further performed a whole genome sequencing parallel to the ribonucleic acid sequence on the new coronavirus infection samples
.

The sequencing coverage is about 30 times, and more than 95% of the mosaic events cover at least 10 times.

Whole genome sequencing of new coronavirus infection samples

Whole genome sequencing of novel coronavirus infection samples Whole genome sequencing of novel coronavirus infection samples

Finally, in order to verify that the source of the chimeric events in the transcriptome sequencing results was the introduction of random connections during the library construction process, the researchers mixed the RNA of the neocoronavirus-infected cells and uninfected zebrafish embryos to construct an RNA library and further perform RNA sequencing
.

The results showed that in addition to the expected human-virus chimeric RNA fragments, there are also zebrafish-virus chimeric RNA fragments, and the ratio of virus chimeric reads in humans to zebrafish is proportional to the ratio of human to zebrafish reads Related

Chimeric events may come from library construction, but are not natural events

Chimeric events may come from library construction, but not natural events Chimeric events may come from library construction, but not natural events

In short, this study ruled out the ability of the novel coronavirus RNA to integrate into the host genome through systematic analysis of transcriptome sequencing, whole genome sequencing, and mixed sample transcriptome sequencing

Original source:

Original source:

Chen, YS.

Comprehensive analysis of RNA-seq and whole genome sequencing data reveals no evidence for SARS-CoV-2 integrating into host genome in this message