Sci Trans Med: CRISPR edits human fat cells to prevent diet-induced obesity.

Obesity and metabolic syndrome are currently increasing rapidly worldwide, leading to high morbidity and mortality.
strategies for the prevention and treatment of obesity and its complications are very important to the health care community.
in mammals, both brown adipose tissue (BAT) and white adipose tissue (WAT) contribute to a systematic energy balance;
WAT is the main place to store excess fuel, containing a single fat droplet, while BAT is a specific energy consumption site, with multiple fat droplets.
previous studies have shown that activating BAT increases energy consumption, and its activity is inversely inversely related to body mass index and fat mass, making BAT an attractive target for anti-obesity therapies.
, however, the effectiveness of cell-based therapy in humans remains unclear.
recently, researchers published a paper in the journal Translation Scienceal Medicine, which uses CRISPR-Cas9-SAM-gRNA to activate the expression of endogenetic decouple protein 1 in human white fat pregenuity cells to create human brown fat-like cells (HUMBLE).
To see if HUMBLE cells could prevent metabolic disorders in mice in diet-induced obesity, the researchers monitored the metabolic esophoids of recipient mice fed a 45% high-fat diet (HFD) 2 weeks before transplantation and continued the same diet for 4 weeks after transplantation.
who received HUMLE and brown controlled fat cells gained less weight than 100 mice that received white controlled fat cells.
mice receiving HUMBLE or brown controlled fat cell transplants also improved glucose tolerance and insulin sensitivity by about 30 to 35 percent compared to mice receiving white controlled fat cells.
there was no statistically significant change in serum insulin, but the concentrations of circulating triglycerides in humble and brown control fat cell groups were lower than in mice receiving white controlled fat cell transplants.
mice that transplanted HUMLE or brown controlled fat cells also consumed more oxygen and produced more calories in the dark cycle than mice that received white control fat cells, and there was no difference in food intake between the three.
compared to mice receiving white controlled fat cell transplants, HUMBLE or brown controlled fat cell transplants kept mice with higher core body temperature during cold exposure.
researchers also observed an improvement in the metabolic esotypes of HUMLE cell transplantation in six separate queues, each with six to eight mice.
It is worth noting that white control fat cell transplantation had no effect on weight, glucose tolerance, oxygen consumption, or heat production compared to mice that transplanted the substitin gum alone, indicating that white control fat cell transplantation had no negative effect on metabolism.
From a mechanism point of view, the increase in arginine/nitric oxide (NO) metabolism in HUMBLE fat cells promotes the production of NO in red blood cells carried by S-nitrosol and nitrite to activate endogenetic brown fat and improve glucose balance in the affected animals.
data together demonstrate that the use of CRISPR-Cas9 technology to engineer white fat cells in humans can result in brown fat esother esopes and may open up cell-based treatment opportunities for the fight against obesity and diabetes.
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