Science Advances: Studies have confirmed that cfDNA contains tumor-specific TF binding information, which can be used to map tumor regulation using plasma

The interaction of transcription factors (TFs) with DNA is fundamental to gene regulation, and different cell types are defined by unique TF binding profiles

cfDNA has the potential to map tumor epigenomes in real time and therefore can help reveal the regulation

Recently, a research team from the University of Colorado in the United States mapped the TF binding map of individual binding sites in plasma cfDNA, defining cfDNA characteristics

The article was published on Science Advances

ChIP-seq and CUT&RUN methods applied to cell line and tissue samples are standard methods for detecting TFBS in the genome

Figure 1.

Figure 2: Lymphatic/myeloid-derived cfDNA contains a hematopoietic TF footprint

Figure 3: The ER+ breast cancer xenograft model is able to recognize pure tumor cfDNA imprinting

Figure 6: Tissue-specific TFBSs enable detection of disease states

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Transcription factor–nucleosome dynamics from plasma cfDNA identifies ER-driven states in breast cancer.