【Science Sub-Journal】Single-cell sequencing for male infertility—optimizing circular sperm cell injection technology

This article is original from Translational Medicine Network, please indicate the source for reprinting 

Author: kope

Introduction: Azoospermia is the main cause of male infertility, and its incidence is about 1% of the total male population

Recently, Professor Bai Xiaochun, Professor Zhao Xiaoyang and other research teams from the School of Basic Medicine of Southern Medical University published relevant research results in the journal "Science Advance"

sperm cells

 01 

Round spermatocyte injection (ROSI) is a technique in which the precursor cells (round spermatids) of mature sperm removed from semen or testis are injected into an egg to facilitate conception

The injection of round sperm cells into the egg usually fails to activate the egg, and only when the egg is activated can the egg complete the second meiosis and normal fertilization, indicating that mature sperm are not only characterized by vitality and swimming, but also need to be able to induce calcium ion shocks , activate the egg

single-cell sequencing

 02 

With the advent of single-cell sequencing methods, especially those based on single-cell multi-omics sequencing, it is now possible to simultaneously decipher complex regulatory networks that control different molecular layers at single-cell resolution

The researchers also identified reprogramming defects in ROSI embryos that were associated with misexpression (downregulation) of a set of minor ZGA genes

new optimization scheme

 03 

The researchers used a single-cell multi-omics sequencing approach to characterize the transcriptome, chromatin accessibility and DNA methylation landscape of mouse ROSI embryos from early round spermatozoa

While most of these modifications are resolved after fertilization, escape of parental bias during reprogramming is critical for establishing gene-specific expression patterns

References:

This article is intended to introduce the progress of medical research and cannot be used as a reference for treatment plans

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