Why not skip RNA purification for gene expression analysis?

For gene expression analysis, reverse transcription plus quantitative PCR (RT-qPCR) is a very classic method

With the widespread application of high-throughput gene expression analysis, such as genome-wide RNAi and compound library screening, people look forward to simple methods that can directly perform gene expression analysis on 96-well cell cultures without the need for cell collection and RNA purification

SingleShot™ is the name of this series of kits

The SingleShot™ Cell Lysis Kit can process samples from as low as 10 cells and up to 100,000 cells, completely removing genomic DNA while preserving the integrity of RNA, avoiding the loss of transcripts caused by column purification and allowing gene expression The results have excellent repeatability and accuracy

As Paul Streng, senior product manager of Bio-Rad, said, the SingleShot kit is very suitable for those high-throughput laboratories with a large workload, and those researchers who have a limited number of cells and need accurate analysis

The SingleShot kit also includes an RNA control, allowing researchers to determine the optimal cell number and initial amount of RT-qPCR reaction

The SingleShot series products not only include a single cell lysis kit, but also include one-step and two-step RT-qPCR kits, which are compatible with the SYBR Green method and the probe method, respectively

Request details of SingleShot cell lysis kit now

Bio-Rad also released a technical guide for the SingleShot SYBR Green Kit to evaluate its performance and compare it with other products

Technical guide:

Accurate and Reproducible RT-qPCR Gene Expression Analysis on Cell Culture Lysates Using the SingleShot™ SYBR® Green Kit, Rev A