With the help of blood direct expansion, NEB Q5 series new products are coming!

There are many kinds of PCR inhibitors in blood, including heme, protein, salt and anticoagulant, and general polymerase is not suitable for direct gene detection in blood

Therefore, when blood samples are amplified by PCR, DNA extraction and purification are required first, which undoubtedly becomes the key to affecting the efficiency of PCR detection

Today, the editor will introduce to you a new blockbuster NEB product:

NEB Q5® Hematogenous Direct Expansion 2X Master Mix (M0500)

Human whole blood/dried blood spot sample direct amplification

1 Product Features

▪ Can directly use human whole blood cells or dried blood spot samples for amplification without DNA purification

2 Product Information

▪ This master mix contains Q5® Hot Start Ultra-Fidelity DNA Polymerase, a thermostable DNA polymerase with 3'→5' exonuclease activity that provides robust DNA amplification with low error rates

A Tm Calculator is recommended to confirm the optimal annealing temperature of primers for the polymerase/buffer of interest

3 Product performance display

3.

According to the standard reaction conditions recommended by the manufacturer's instructions, use Q5® Blood Source Direct Amplification 2X Master Mix for 35 cycles of PCR amplification

A.

B.

3.

Different GC content (23-77%) (panel A) and different lengths (2-7 kb) (panel B) using Q5® Blood-derived Direct Amplification 2X Master Mix and several other commercially available blood direct-amplification polymerases, respectively.

3.

PCR was performed on the human genomic target (604 bp) in dried blood using Q5® Blood Direct Amplification 2X Master Mix according to the standard reaction conditions recommended by the manufacturer's instructions

Today's new product introduction is here.
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