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How-to guide for lyovirus use
Time of Update: 2021-01-20
The third day, change the culture fluid: generally after 24 years of childhood will contain lysovirus culture liquid replaced with normal culture fluid, after infection to observe the state of the cell, if the lyovirus has a significant toxic effect on the cell and affect the cell growth state, can be as short as the virus 4 childhood replacement of fresh culture solution continued culture (recommended in 8-12 hours replacement is appropriate).
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Testing of fecal colium
Time of Update: 2021-01-20
2． 44 degrees C lactose fermentation test the sample inoculated with sterile operation in the lactose bile salt fermentation tube (1 mL or more 1 mL and 1 mL or less, with a single lactose bile salt fermentation tube), placed (44 soil 0.5) in the water bath, culture (24 soil 2) h.
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The PCR method detects myosomes
Time of Update: 2021-01-20
(2) template production: in sterile conditions, take cell culture on clear 1 00ul in a sterile 0.5 ml plastic centrifuge tube, covered with a lid, 95 degrees C water bath heating 5min.
(6) takes a centrifugal tube containing the above reaction system and adds 5ul deionized water as a negative control tube.
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Vaccination, separation purification and culture techniques
Time of Update: 2021-01-20
, vaccination microorganisms to the artificial culture suitable for its growth and reproduction or the process of living organisms is called inoculation.
This method is to inoculate a small number of microorganisms on the surface of the plate, into three points of egaldiscular triangles, so that it independently forms bacteria backward, to observe and study their form.
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The definition and determination significance of E. coli
Time of Update: 2021-01-20
In food hygiene microbial testing, can be used as an indicator of fecal contamination bacteria based on the above conditions, the largest number of feces is colium, and E.
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Yeast indica PCR method
Time of Update: 2021-01-20
Ask: very depressed, recently doing electro-turned-bi-chi yeast, but the conversion did not have a suitable screening method, if the genome is time-time-saving, and because my chances of this electri
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The preparation of LB cultures
Time of Update: 2021-01-20
LB culture base preparation: ingredients tryptone 10g yeast extract 5g sodium chloride (NaCl) 10g Solid media plus agar powder 15-20g plus double steamed water to 1000mL, pH to 7.2,121C sterilization 30min preparation method (1) weighing the required amount of tryplin , yeast extract and NaCl, respectively, placed in berries.
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Phage culture
Time of Update: 2021-01-20
the phage body progenitor 100 sl is evenly mixed with the host bacteria suspension liquid for 300 sl and remained in place for 15 minutes to infect it.
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How to take responsibility for your own health starts with improving our gut
Time of Update: 2021-01-12
Probiotics and probiotics complement each other and play a common role in promoting the growth and reproduction of beneficial bacteria, inhibiting the growth of harmful bacteria, maintaining the balance of the micro-ecological environment of the intestine, preventing and improving constipation and diarrhea, improving immunity and maintaining human health.
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Genome-wide correlation analysis found that acetic kinases and peptide enzymes were hydrolyzed with the Swiss Lactobacillus protein
Time of Update: 2021-01-12
。 Original title: Whole Genome Association Analysis Finds Acetic Kinase and Peptide Enzymes Linked to Swiss Lactobacillus Protein Hydrolysis Capacity Recently, the Key Laboratory of the Ministry of Dairy Biotechnology and Engineering of Inner Mongolia Agricultural University was published in Food Microbiology, a top journal of food microbiology Academic paper entitled "Acetate kinase and peptidases are associated with the proteolytic activity Lactobacillus helveticus isolated from fermented food".
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Don't let stress ruin your gut and overall health
Time of Update: 2021-01-12
is also worth noting that simple stress interventions, such as exercise and meditation, have been shown to reduce inflammation and improve the quality of life of people with inflammatory bowel disease.
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Health care should be taken seriously by people of all ages
Time of Update: 2020-12-31
But if you also eat a lot of carbohydrates by the age of 50, the body's sugar metabolism pattern will always remain active, while mitochondrial metabolism mode is relatively weak, at this time the intake of oxygen will be converted to reactive oxygen, causing some damage to the intestines.
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Cold winter health attention to what
Time of Update: 2020-12-31
Pay attention to keep warm to reduce hot and cold sudden stimulation from the warm room with heating, suddenly walked to the cold wind bone-chilling outdoor, which is inevitable in winter, but do a good job of keeping warm measures to reduce the sudden stimulation of hot and cold, is to reduce the risk of sudden cardiovascular disease is an important aspect.
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Hydrogen peroxide released from the inner wall of the colon protects the gut bacterium
Time of Update: 2020-12-31
a recent study published in Cell Host and Microbe, a team led by the University of California, Davis, found that an enzyme in the colon's inner wall releases hydrogen peroxide to protect gut microbiota.
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Distance produces beauty! Cell Sub-Journal: Intestinal-produced "sterilizing fluid" is maintaining health
Time of Update: 2020-12-22
a recent study published in Cell Host and Microbe, a team led by the University of California, Davis, found that an enzyme in the colon's inner wall releases hydrogen peroxide to protect gut microbiota.
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Pain, itchy field of the overall topic of thinking design
Time of Update: 2020-12-22
The difference is that pain stimulation can cause immediate pain and reflexive avoidance, while itch stimulation can cause long feelings such as the loss of the moon and scratching behavior with no time accuracy.
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Probiotics to protect children's health
Time of Update: 2020-12-22
fourth, according to the characteristics of breast milk and national regulations, infants and young children to choose the appropriate strain: the study found that breast-feeding infants in the intestines of probiotics mainly Bifidobacteria, about 60% to 90%.
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Based on the principle and choice of different gene regulation methods of viral vectors
Time of Update: 2020-12-22
In gene knockout, Cas9 protein under the guidance of gRNA, the specific DNA bits are cut to produce double-stranded fractures, and then the cells through non-isocent end connections to repair the DNA, in the process, will randomly cause the loss or increase of the base, the gene code mutation , resulting in the removal of the encoded gene.
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The design of the overall topic in the field of stroke
Time of Update: 2020-12-22
stroke project design 1, building animal models (MCAO molding, ICH molding, TBI molding, etc.) MCAO molding: MCAO is an acronym for middle cerebral artery occlusion, Chinese meaning middle brain artery embolism.
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CRISPR/Cas9 gRNA library screens functional genes
Time of Update: 2020-12-22
CRLSPR/Cas9 is an accessary immune system used by bacteria and paleontogens to resist genetic material invasions such as foreign Using THERLSPR/Cas9 technology to establish a full group mutant library of mammalian > genes or a library of gene mutants associated with certain functions, through functional screening and a collection, and subsequent amplification and in-depth sequencing analysis, to identify genes associated with screening esotypes, called CRLSPR/Cas9 gRNA library.
